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	Facile fabrication of biomimetic chitosan membrane with honeycomb-like structure for enrichment of glycosylated peptides
	Zhang, Luwei1,2; Ma, Shujuan1,2; Chen, Yao2,3; Wang, Yan1,2; Ou, Junjie2; Uyama, Hiroshi1,4; Ye, Mingliang2
刊名	Analytical chemistry
	2019-02-19
卷号	91期号:4页码:2985-2993
ISSN号	0003-2700
DOI	10.1021/acs.analchem.8b05215
通讯作者	Ou, junjie(junjieou@dicp.ac.cn) ; Uyama, hiroshi(uyama@chem.eng.osaka-u.ac.jp)
英文摘要	In the study of glycoproteomics with mass spectrometry, certain pretreatments of samples are required for eliminating the interference of nonglycopeptides and improving the efficiency of glycopeptides detection. although hydrophilic interaction chromatography (hilic) has been developed for enrichment of glycosylated peptides, a plethora of hydrophilic materials always suffered from large steric hindrance, great cost, and difficulty with modifications of high-density hydrophilic groups. in this work, a 1 mm thick biomimetic honeycomb chitosan membrane (bhcm) with honeycomb-like accessible macropores was directly prepared by the freeze-casting method as an adsorbent for hilic. the n-glycopeptides from trypsin digests of immunoglobulin g (igg), mixture of igg and bovine serum albumin (bsa), and serum proteins were enriched using this material and compared with a commercial material zic-hilic. the biomimetic membrane could identify as many as 32 n-glycopeptides from the igg digest, exhibiting high sensitivity (about 50 fmol) and a wide scope for glycopeptide enrichment. a molar ratio of igg trypsin digest to bovine serum albumin trypsin digest as low as 1/500 verified the outstanding specificity and efficiency for glycopeptide enrichment. in addition, 270 unique n-glycosylation sites of 400 unique glycopeptides from 146 glycosylated proteins were identified from the triplicate analysis of 2 mu l human serum. furthermore, 48 unique o-glycosylation sites of 278 unique o-glycopeptides were identified from the triplicate analysis of 30 mu g deglycosylated fetuin digest. these results indicated that the chitosan-based membrane prepared in this work had great potential for pretreatment of samples in glycoproteomics.
WOS关键词	MESOPOROUS SILICA MATERIALS ; HIGHLY SPECIFIC ENRICHMENT ; MASS-SPECTROMETRY ; EFFICIENT ENRICHMENT ; SELECTIVE ENRICHMENT ; N-GLYCOPEPTIDES ; GLYCOMICS ; IDENTIFICATION ; GLYCOPROTEINS ; COMPOSITES
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000459642400058
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372758
专题	大连化学物理研究所
通讯作者	Ou, Junjie; Uyama, Hiroshi
作者单位	1.Northwest Univ, Key Lab Synthet & Nat Funct Mol Chem, Natl Demonstrat Ctr Expt Chem Educ, Minist Educ,Coll Chem & Mat Sci, Xian 710127, Shaanxi, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan
推荐引用方式 GB/T 7714	Zhang, Luwei,Ma, Shujuan,Chen, Yao,et al. Facile fabrication of biomimetic chitosan membrane with honeycomb-like structure for enrichment of glycosylated peptides[J]. Analytical chemistry,2019,91(4):2985-2993.
APA	Zhang, Luwei.,Ma, Shujuan.,Chen, Yao.,Wang, Yan.,Ou, Junjie.,...&Ye, Mingliang.(2019).Facile fabrication of biomimetic chitosan membrane with honeycomb-like structure for enrichment of glycosylated peptides.Analytical chemistry,91(4),2985-2993.
MLA	Zhang, Luwei,et al."Facile fabrication of biomimetic chitosan membrane with honeycomb-like structure for enrichment of glycosylated peptides".Analytical chemistry 91.4(2019):2985-2993.

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