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	The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material
	Luo, Jian1; Li, Qian1; Sun, Xitong2; Tian, Jing1; Fei, Xu1; Shi, Feng1; Zhang, Nan1; Liu, Xiangli1
刊名	Rsc advances
	2019
卷号	9期号:8页码:4514-4520
ISSN号	2046-2069
DOI	10.1039/c9ra00075e
通讯作者	Li, qian(liqian19820903@163.com) ; Sun, xitong(sunxt@dicp.ac.cn)
英文摘要	Silica material has high specific surface area and excellent chemical stability, which make it useful for enzyme immobilization. in this work, naringinase was immobilized from fermentation broth of aspergillus niger ffcc uv-11 by silica materials with different pore diameters of 2 nm (mcm-41), 7.7 nm (sba-15) and 80 nm (silica gel). it was shown that sba-15 had the highest naringinase activity, and this was chosen as a suitable carrier material for naringinase immobilization. first, sba-15 was modified by glutaraldehyde at a concentration of 7% at 25 degrees c for 2 h, and it was then used for the immobilization of naringinase. at ph 3.5, the immobilized naringinase activity reached 467.62 u g(-1) at 40 degrees c for 4 h when the initial naringinase activity was 89.04 u ml(-1). furthermore, at the optimal reaction temperature of 45 degrees c and ph of 4.5, the binding efficiency, activity recovery rate and specific activity of the immobilized naringinase were 63.66%, 87.64% and 517.43 u g(-1), respectively. compared with free naringinase, in naringin hydrolysis, the immobilized naringinase performed over a wide ph application range and had good thermal stability. even more important, the immobilized naringinase retained 61.81% of the residual naringinase activity after eight consecutive cycles, and kept 80.95% of the residual naringinase activity after one month of storage. this study provides an ideal carrier material and some basic data for naringinase immobilization technology, which will greatly promote the application of naringinase in industrial fruit juice processing.
WOS关键词	CATALYTIC-ACTIVITY ; MESOPOROUS SILICA ; NANOPARTICLES
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000459896700047
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372704
专题	大连化学物理研究所
通讯作者	Li, Qian; Sun, Xitong
作者单位	1.Dalian Polytech Univ, Dept Bioengn, Dalian 116034, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Luo, Jian,Li, Qian,Sun, Xitong,et al. The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material[J]. Rsc advances,2019,9(8):4514-4520.
APA	Luo, Jian.,Li, Qian.,Sun, Xitong.,Tian, Jing.,Fei, Xu.,...&Liu, Xiangli.(2019).The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material.Rsc advances,9(8),4514-4520.
MLA	Luo, Jian,et al."The study of the characteristics and hydrolysis properties of naringinase immobilized by porous silica material".Rsc advances 9.8(2019):4514-4520.

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