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	Dispersive matrix solid-phase extraction method coupled with high performance liquid chromatography-tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute plants and its application for geographical distribution, study
	Li, Yuxuan1,2; Deng, Ting1,2; Duan, Chunfeng1; Ni, Lanxiu1,2; Wang, Nan1,2; Guan, Yafeng1
刊名	Journal of agricultural and food chemistry
	2019-03-13
卷号	67期号:10页码:3037-3045
关键词	Dispersive matrix solid-phase extraction Endogenous brassinosteroids Oilseed rape Arabidopsis thaliana seedlings Geographical distribution
ISSN号	0021-8561
DOI	10.1021/acs.jafc.8b07224
通讯作者	Duan, chunfeng(cfduan@dicp.ac.cn) ; Guan, yafeng(guanyafeng@dicp.ac.cn)
英文摘要	An ultrasensitive analysis method for quantification of endogenous brassinosteroids in fresh minute plants was developed based on dispersive matrix solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry. during the dispersive matrix solid-phase extraction, plant samples were first ground with solid sorbent (dispersant) in one microcentrifuge tube and then centrifuged after adding extraction solvent and cleanup materials (another type of sorbent). three protocols based on dispersive matrix solid-phase extraction were compared and discussed for plant samples with different matrix complexity. the choice of any protocol was a compromise of increasing purification efficiency and decreasing sample loss. under optimized conditions, the limits of detection were 1.38-6.75 pg ml(-1) for five brassinosteroids in the oilseed rape samples. the intraday and interday precisions were in the range of 0.8%-9.8% and 4.6%-17.3%, respectively. the proposed method was successfully applied to detection of endogenous brassinosteroids in milligram oilseed rape (2.0 mg) and submilligram arabidopsis thaliana seedlings (0.5 mg). finally, the geographical distribution of five endogenous brassinosteroids of brassica napus l. oilseed rape in different provinces of origin in the yangtze river basin was described.
WOS关键词	TRACE DETERMINATION ; SAMPLE PREPARATION ; MICROEXTRACTION ; SORBENT ; PHYTOHORMONES ; GIBBERELLINS ; RESIDUES ; HORMONES ; CADMIUM ; TISSUES
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000461538100033
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2372677
专题	大连化学物理研究所
通讯作者	Duan, Chunfeng; Guan, Yafeng
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, 457 Zhongshan Rd, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Yuxuan,Deng, Ting,Duan, Chunfeng,et al. Dispersive matrix solid-phase extraction method coupled with high performance liquid chromatography-tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute plants and its application for geographical distribution, study[J]. Journal of agricultural and food chemistry,2019,67(10):3037-3045.
APA	Li, Yuxuan,Deng, Ting,Duan, Chunfeng,Ni, Lanxiu,Wang, Nan,&Guan, Yafeng.(2019).Dispersive matrix solid-phase extraction method coupled with high performance liquid chromatography-tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute plants and its application for geographical distribution, study.Journal of agricultural and food chemistry,67(10),3037-3045.
MLA	Li, Yuxuan,et al."Dispersive matrix solid-phase extraction method coupled with high performance liquid chromatography-tandem mass spectrometry for ultrasensitive quantification of endogenous brassinosteroids in minute plants and its application for geographical distribution, study".Journal of agricultural and food chemistry 67.10(2019):3037-3045.

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