Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'

doi:10.3389/fpls.2017.02017

	Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'
	Zhang, Xiaoqian 1,2,3; Li, Kecheng 1,2; Xing, Ronge1 ; Liu, Song 1; Li, Pengcheng1,2
刊名	FRONTIERS IN PLANT SCIENCE
	2017-11-28
卷号	8
关键词	Chitosan Metabolic Profiling Carbon Metabolism Nitrogen Assimilation Wheat Seedlings
DOI	10.3389/fpls.2017.02017
文献子类	Article
英文摘要	Chitosan plays an important role in regulating growth and eliciting defense in many plant species. However, the exact metabolic response of plants to chitosan is still not clear. The present study performed an integrative analysis of metabolite profiles in chitosan-treated wheat seedlings and further investigated the response of enzyme activities and transcript expression related to the primary carbon (C) and nitrogen (N) metabolism. Metabolite profiling revealed that chitosan could induce significant difference of organic acids, sugars and amino acids in leaves of wheat seedlings. A higher accumulation of sucrose content was observed after chitosan treatment, accompanied by an increase in sucrose phosphate synthase (SPS) and fructose 1, 6-2 phosphatase (FBPase) activities as well as an up-regulation of relative expression level. Several metabolites associated with tricarboxylic acid (TCA) cycle, including oxaloacetate and malate, were also improved along with an elevation of phosphoenolpyruvate carboxylase (PEPC) and pyruvate dehydrogenase (PDH) activities. On the other hand, chitosan could also enhance the N reduction and N assimilation. Glutamate, aspartate and some other amino acids were higher in chitosan-treated plants, accompanied by the activation of key enzymes of N reduction and glutamine synthetase/glutamate synthase (GS/GOGAT) cycle. Together, these results suggested a pleiotropic modulation of carbon and nitrogen metabolism in wheat seedlings induced by chitosan and provided a significant insight into the metabolic mechanism of plants in response to chitosan for the first time, and it would give a basic guidance for the future application of chitosan in agriculture.
语种	英语
WOS记录号	WOS:000416309800001
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143294]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiaoqian,Li, Kecheng,Xing, Ronge,et al. Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'[J]. FRONTIERS IN PLANT SCIENCE,2017,8.
APA	Zhang, Xiaoqian,Li, Kecheng,Xing, Ronge,Liu, Song,&Li, Pengcheng.(2017).Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'.FRONTIERS IN PLANT SCIENCE,8.
MLA	Zhang, Xiaoqian,et al."Metabolite Profiling of Wheat Seedlings Induced by Chitosan: Revelation of the Enhanced Carbon and Nitrogen Metabolism'".FRONTIERS IN PLANT SCIENCE 8(2017).