Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles

doi:10.1007/s00604-017-2463-1

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	Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles
	Zeng, Yan1 ; Zhang, Dun 1; Qi, Peng1 ; Zheng, Laibao1,2
刊名	MICROCHIMICA ACTA
	2017-12-01
卷号	184 期号:12 页码:4809-4815
关键词	Dnadetection Goldnanoparticle Colorimetry Self-assembly Dna Nanostructure
DOI	10.1007/s00604-017-2463-1
文献子类	Article
英文摘要	The authors describe a strategy for colorimetric detection of DNA. It is making use of a target catalyzed DNA nanostructure assembly and gold nanoparticles. The assay comprises the following steps: (a) A programmed DNA nanostructure is assembled from three auxiliary hairpin structure DNAs and the single stranded DNA (ssDNA; the target/analyte); (b) in the presence of target DNA, these three hairpin DNAs are opened, thereby activating a catalytic self-assembly process via a target assisted toe-hold strand displacement reaction; (c) The formed DNA nanostructures are mixed with gold nanoparticles. As the DNA nanostructure is less stabilized without ssDNA sticky ends, it cannot prevent the gold nanoparticles (AuNPs) to undergo salt-induced aggregation which is accompanied by a color change from purple to blue; (d) The color change of the colloid solution can be read out with bare eyes or instrumentally. The detection limit by using UV-vis spectrometry is 0.6 pM of target DNA. This is comparable to previously AuNP-based methods. Thus, this assay provides free modification detection based on DNA nanostructure without sophisticated procedures. Conceivably, the method can be applied to numerous other DNA targets.
语种	英语
WOS记录号	WOS:000414547400029
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143036]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, 19 Jia Yuquan Rd, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Yan,Zhang, Dun,Qi, Peng,et al. Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles[J]. MICROCHIMICA ACTA,2017,184(12):4809-4815.
APA	Zeng, Yan,Zhang, Dun,Qi, Peng,&Zheng, Laibao.(2017).Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles.MICROCHIMICA ACTA,184(12),4809-4815.
MLA	Zeng, Yan,et al."Colorimetric detection of DNA by using target catalyzed DNA nanostructure assembly and unmodified gold nanoparticles".MICROCHIMICA ACTA 184.12(2017):4809-4815.