Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro

doi:10.1016/j.carbpol.2005.04.007

	Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro
	Xing, RE; Liu, S ; Yu, HH; Guo, ZY ; Li, Z ; Li, PC
刊名	CARBOHYDRATE POLYMERS
	2005-08-04
卷号	61 期号:2 页码:148-154
关键词	Chitosans High-molecular Weight High-sulfate-content Dichloroacetic Acid Formic Acid Reducing Power Chelating Activity Radical Scavenging Effect
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2005.04.007
文献子类	Article
英文摘要	The influence of molecular weight and substitution degree of sulfated polysaccharides on their biological activity is considered in majority of works involving the anticoagulant or antiviral properties of these substances. Therefore, the present paper describes the effect of preparation conditions of sulfated chitosans on their molecular weight and sulfur content, such as different reaction time, acid solvent and temperature. Foregoing literature expounded the action of dichloroacetic acid (DCAA) as acid solvent in homogeneous reaction. However, DCAA is expensive and noxious, therefore, in the present paper cheap and non-noxious formic acid (88%) was in place of DCAA. Furthermore, during reaction formic acid was not dehydrated. Under formic acid we obtained the satisfying results that was higher yield and equivalent sulfur contents compared to DCAA. IR and (13)C NMR spectrums proved the structure of the resultant obtained under formic acid or DCAA to be same. Now, it has not been reported for formic acid as acid solvent in homogeneous reaction of chitosan sulfatation. In this present paper, we also determined antioxidant activity of high-molecular weight and high-sulfate-content chitosans (HCTS). The results showed that HCTS could scavenge superoxide and hydroxyl radical. Its IC(50) is 0.012 and 3.269 mg/mL, respectively. It had obviously reducing power and slight chelating activity. The data obtained in in vitro models clearly establish the antioxidant potency of HCTS. It is a potential antioxidant in vitro. (C) 2005 Elsevier Ltd. All rights reserved.; The influence of molecular weight and substitution degree of sulfated polysaccharides on their biological activity is considered in majority of works involving the anticoagulant or antiviral properties of these substances. Therefore, the present paper describes the effect of preparation conditions of sulfated chitosans on their molecular weight and sulfur content, such as different reaction time, acid solvent and temperature. Foregoing literature expounded the action of dichloroacetic acid (DCAA) as acid solvent in homogeneous reaction. However, DCAA is expensive and noxious, therefore, in the present paper cheap and non-noxious formic acid (88%) was in place of DCAA. Furthermore, during reaction formic acid was not dehydrated. Under formic acid we obtained the satisfying results that was higher yield and equivalent sulfur contents compared to DCAA. IR and C-13 NMR spectrums proved the structure of the resultant obtained under formic acid or DCAA to be same. Now, it has not been reported for formic acid as acid solvent in homogeneous reaction of chitosan sulfatation. In this present paper, we also determined antioxidant activity of high-molecular weight and high-sulfate-content chitosans (HCTS). The results showed that HCTS could scavenge superoxide and hydroxyl radical. Its IC50 is 0.012 and 3.269 mg/mL, respectively. It had obviously reducing power and slight chelating activity. The data obtained in in vitro models clearly establish the antioxidant potency of HCTS. It is a potential antioxidant in vitro. (C) 2005 Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
URL标识	查看原文
语种	英语
WOS记录号	WOS:000231168000004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3543]
专题	海洋研究所_海洋生物技术研发中心海洋研究所_海洋环境工程技术研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xing, RE,Liu, S,Yu, HH,et al. Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro[J]. CARBOHYDRATE POLYMERS,2005,61(2):148-154.
APA	Xing, RE,Liu, S,Yu, HH,Guo, ZY,Li, Z,&Li, PC.(2005).Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro.CARBOHYDRATE POLYMERS,61(2),148-154.
MLA	Xing, RE,et al."Preparation of high-molecular weight and high-sulfate content chitosans and their potential antioxidant activity in vitro".CARBOHYDRATE POLYMERS 61.2(2005):148-154.