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	Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)
	Ran, Tao1,2; Mei, Liu1; Lei, Wang1; Aihua, Liu3; Ru, He4; Jie, Sun1; Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China
刊名	AQUACULTURE RESEARCH
	2009-08-20
卷号	40期号:12页码:1350-1357
关键词	Aquatic Feed Gm Soybean Nested-pcr Oreochromis Niloticus Tissues Transfer
ISSN号	1355-557X
DOI	10.1111/j.1365-2109.2009.02187.x
文献子类	Article
英文摘要	We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10(-10) g mu L(-1) DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non-GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal.; We used nested-polymerase chain reaction (PCR) to detect Roundup Ready soybean in aquatic feeds and feeding tilapias. A template concentration of 10(-10) g mu L-1 DNA solution could be detected with a dilute degree of 0.01%. Most (90.6%) of the aquatic feeds containing soybean byproduct included exogenous DNA segments. We also compared genetically modified (GM) soybean with non-GM soybean diets in feeding tilapias (Oreochromis niloticus, GIFT strain) and examined the residual fragments (254 bp) of GM soybeans. Tilapias receiving GM soybean diets had DNA fragments in different tissues and organs, indicating that exogenous GM genes were absorbed systemically and not completely degraded by the tilapia's alimentary canal.
学科主题	Fisheries
URL标识	查看原文
语种	英语
WOS记录号	WOS:000269056300002
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1869]
专题	海洋研究所_海洋生物技术研发中心
通讯作者	Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Rongcheng Entry Exit Inspect & Quarantine Bur, Rongcheng, Peoples R China 3.Qingdao Entry Exit Inspect & Quarantine Bur, Qingdao, Peoples R China 4.Haerbin Inst Technol, Weihai, Peoples R China
推荐引用方式 GB/T 7714	Ran, Tao,Mei, Liu,Lei, Wang,et al. Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)[J]. AQUACULTURE RESEARCH,2009,40(12):1350-1357.
APA	Ran, Tao.,Mei, Liu.,Lei, Wang.,Aihua, Liu.,Ru, He.,...&Mei, L, Chinese Acad Sci, Ctr Marine Biotechnol, Inst Oceanol, 7th Nanhai Rd, Qingdao 266071, Peoples R China.(2009).Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready).AQUACULTURE RESEARCH,40(12),1350-1357.
MLA	Ran, Tao,et al."Detection of transgenic DNA in tilapias (Oreochromis niloticus, GIFT strain) fed genetically modified soybeans (Roundup Ready)".AQUACULTURE RESEARCH 40.12(2009):1350-1357.

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