Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system

	Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system
	Yu, Linfen 1; Shen, Zheng 1; Mo, Jiankun 2; Dong, Xiuling 3; Qin, Jianhua 1; Lin, Bingcheng 1
刊名	electrophoresis
	2007-12-01
卷号	28 期号:24 页码:4741-4747
关键词	cell electrophoresis K562 cells microfluidic chip multipoint detection
通讯作者	林炳承 ; 秦建华
产权排序	1;1
英文摘要	in this work, the electrophoretic mobility (epm) measurement of individual cells was investigated by a simple on-chip electrophoresis system with lif multipoint detection. the system enabled the characterization of cell electrophoresis behavior as well as the fluorescence signal from individual cells simultaneously. the measurement yielded the electropherograms of a large number of cells labeled with dye, in which the migration time and migration distance could be obtained easily. the epm has been demonstrated to be different between the k562 cells and k562 cells treated with anticancer drug arsenic trioxide (as(2)o(3)). the k562 cells were found to exhibit a lower epm compared to the cells after drug addition with different concentration. in this preliminary study, over 300 cells could be analyzed within 2 h, demonstrated a much higher analysis throughput compared with traditional methods. the established system is simple and fast, which is expected to be a promising method for evaluating cell surface properties and to be useful in clinical and pharmaceutical applications.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	red-blood-cells ; arsenic trioxide ; apoptosis ; adsorption ; cytometry ; mobility ; flow
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000252465600026
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/99833]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China 2.2nd Peoples Hosp Guangdong Prov, Guangdong, Peoples R China 3.Dalian Third Municipal Hosp, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Yu, Linfen,Shen, Zheng,Mo, Jiankun,et al. Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system[J]. electrophoresis,2007,28(24):4741-4747.
APA	Yu, Linfen,Shen, Zheng,Mo, Jiankun,Dong, Xiuling,Qin, Jianhua,&Lin, Bingcheng.(2007).Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system.electrophoresis,28(24),4741-4747.
MLA	Yu, Linfen,et al."Microfluidic chip-based cell electrophoresis with multipoint laser-induced fluorescence detection system".electrophoresis 28.24(2007):4741-4747.