Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines

doi:10.1016/j.fsi.2017.09.023

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	Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines
	Wang, Xiudan1,4 ; Wang, Mengqiang1 ; Xu, Qingsong 3; Xu, Jiachao1,4 ; Lv, Zhao 1,4; Wang, Lingling 3; Song, Linsheng 2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2017-11-01
卷号	70 页码:308-318
关键词	Crassostrea Gigas Leucine-rich Repeat Domain Immunoglobulin Domain Pattern Recognition Receptor Innate Immunity
DOI	10.1016/j.fsi.2017.09.023
文献子类	Article
英文摘要	Leucine-rich repeat (LRR) domain and immunoglobulin (Ig) domain are both competent immune recognition modules, and the immunological roles of LRR and Ig domain containing-proteins (LRRIGs) are speculated to be multifunctional and worth investigating. In the present study, two novel LRRIGs, CgLRRIG-1 and CgLRRIG-2, were identified and characterized from oyster Crassostrea gigas. Both of them contained an N-terminal LRR region, an Ig domain, a transmembrane region, and a C-terminal cytoplasmic tail. The mRNA transcripts of CgLRRIG-1 and CgLRRIG-2 were constitutively expressed in muscle, gill, hepatopancreas, mantle, gonad and hemocytes with the highest expression level in hepatopancreas. Their mRNA expression levels in hemocytes were significantly up-regulated after the stimulations with four PAMPs including peptidoglycan (PGN), lipopolysaccharide (LPS), glucan (GLU) and polyinosinic-polycytidylic acid (poly I:C) and one bacteria Vibrio anguillarum. The recombinant proteins, rCgLRRIG-1 and rCgLRRIG-2, could bind to PGN, LPS, GLU and poly I:C, and rCgLRRIG-2 exhibited higher binding affinity. Additionally, rCgLRRIG-1 and rCgLRRIG-2 could significantly induce the expression of CgTNF-1 and CgIL17-5 in cultured oyster hemocytes, and the activity of rCgLRRIG-2 was higher than that of rCgLRRIG-1. All these results indicated that CgLRRIG-1 and CgLRRIG-2 could function as immune effectors or pro-inflammatory factors as well as PRRs in oyster. (C) 2017 Elsevier Ltd. All rights reserved.
语种	英语
WOS记录号	WOS:000416186800032
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.qdio.ac.cn/handle/337002/143293]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiudan,Wang, Mengqiang,Xu, Qingsong,et al. Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:308-318.
APA	Wang, Xiudan.,Wang, Mengqiang.,Xu, Qingsong.,Xu, Jiachao.,Lv, Zhao.,...&Song, Linsheng.(2017).Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines.FISH & SHELLFISH IMMUNOLOGY,70,308-318.
MLA	Wang, Xiudan,et al."Two novel LRR and Ig domain-containing proteins from oyster Crassostrea gigas function as pattern recognition receptors and induce expression of cytokines".FISH & SHELLFISH IMMUNOLOGY 70(2017):308-318.