Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii

doi:10.1007/s10811-006-9141-8

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	Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii
	Tan, Cong-Ping ; Zhao, Fang-Qing ; Su, Zhong-Liang ; Liang, Cheng-Wei ; Qin, Song
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2007-08-01
卷号	19 期号:4 页码:347-355
关键词	Chlamydomonas Reinhardtii Chloroplast Transformation Beta-carotene Hydroxylase Gene (Crtr-b) Carotenoids
ISSN号	0921-8971
DOI	10.1007/s10811-006-9141-8
文献子类	Article
英文摘要	A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation.; A carotenoid gene (crtR-B) from the green alga Haematococcus pluvialis, encoding beta-carotene hydroxylase that was able to catalyze the conversion of beta-carotene to zeaxanthin and canthaxanthin to astaxanthin, was cloned into Chlamydomonas reinhardtii chloroplast expression vector p64D to yield plasmid p64DcrtR-B. The vector p64DcrtR-B was transferred to the chloroplast genome of C. reinhardtii using micro-particle bombardment. PCR and Southern blot analyses indicated that crtR-B was integrated into the chloroplast genome of the transformants. RTPCR assays showed that the H. pluvialis crt R-B gene was expressed in C. reinhardtii transformants. The transformants rapidly synthesized carotenoids in larger quantities than the wild-type upon being transferred from moderate to high-intensity white light. This research provides a foundation for further study to elucidate the possible mechanism of photo-protection by xanthophylls and other carotenoids in high light conditions or through exposure to UV radiation.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000248188000006
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5995]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Shandong Inst Light Ind, Coll Food & Bioengn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 4.Ocean Univ Sci & Technol, Coll Chem Engn, Qingdao 266042, Peoples R China
推荐引用方式 GB/T 7714	Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,et al. Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii[J]. JOURNAL OF APPLIED PHYCOLOGY,2007,19(4):347-355.
APA	Tan, Cong-Ping,Zhao, Fang-Qing,Su, Zhong-Liang,Liang, Cheng-Wei,&Qin, Song.(2007).Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii.JOURNAL OF APPLIED PHYCOLOGY,19(4),347-355.
MLA	Tan, Cong-Ping,et al."Expression of beta-carotene hydroxylase gene (crtR-B) from the green alga Haematococcus pluvialis in chloroplasts of Chlamydomonas reinhardtii".JOURNAL OF APPLIED PHYCOLOGY 19.4(2007):347-355.