Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis

doi:10.1016/j.jbiotec.2006.03.010

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	Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis
	Zhang, Jiquan ; Li, Fuhua ; Wang, Zaizhao ; Zhang, Xiaojun ; Zhou, Qian ; Xiang, Jianhai
刊名	JOURNAL OF BIOTECHNOLOGY
	2006-09-01
卷号	125 期号:2 页码:173-184
关键词	Fenneropenaeus Chinensis Ferritin White Spot Syndrome Virus Semi-quantitative Rt-pcr Metal Ions
ISSN号	0168-1656
DOI	10.1016/j.jbiotec.2006.03.010
文献子类	Article
英文摘要	Ferritin, the iron storage protein, plays a key role in iron metabolism. A cDNA encoding ferritin (FcFer) was cloned from hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The predicted protein contains 170 amino acid residues with a predicted molecular weight (MW) about 19, 422.89 Da and theoretical isoelectric point (PI) of 4.73. Amino acid alignment of FcFer revealed 97% homology with Litopenaeus vannamei ferritin. Results of the RT-PCR showed that the expression of FcFer mRNA was up-regulated after shrimp was challenged with either white spot syndrome virus (WSSV) or heavy metal ions (Zn2+ and Cu2+) in the laboratory. A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro. The result of in-gel digestion and identification using LC-ESI-MS showed that two peptide fragments (-DDVALPGFAK- and -LLEDEYLEEQVDS1KK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei ferritin. The recombinant FcFer protein will be proved useful for study on the structure and function of ferritin in F chinensis. (c) 2006 Elsevier B.V. All rights reserved.; Ferritin, the iron storage protein, plays a key role in iron metabolism. A cDNA encoding ferritin (FcFer) was cloned from hepatopancreas of Chinese shrimp, Fenneropenaeus chinensis. The predicted protein contains 170 amino acid residues with a predicted molecular weight (MW) about 19, 422.89 Da and theoretical isoelectric point (PI) of 4.73. Amino acid alignment of FcFer revealed 97% homology with Litopenaeus vannamei ferritin. Results of the RT-PCR showed that the expression of FcFer mRNA was up-regulated after shrimp was challenged with either white spot syndrome virus (WSSV) or heavy metal ions (Zn2+ and Cu2+) in the laboratory. A fusion protein containing FcFer was produced and the purified recombinant protein exhibited similar function of iron uptake in vitro. The result of in-gel digestion and identification using LC-ESI-MS showed that two peptide fragments (-DDVALPGFAK- and -LLEDEYLEEQVDS1KK-) of the recombinant protein were identical to the corresponding sequence of L. vannamei ferritin. The recombinant FcFer protein will be proved useful for study on the structure and function of ferritin in F chinensis. (c) 2006 Elsevier B.V. All rights reserved.
学科主题	Biotechnology & Applied Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000240208800002
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5977]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,et al. Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis[J]. JOURNAL OF BIOTECHNOLOGY,2006,125(2):173-184.
APA	Zhang, Jiquan,Li, Fuhua,Wang, Zaizhao,Zhang, Xiaojun,Zhou, Qian,&Xiang, Jianhai.(2006).Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis.JOURNAL OF BIOTECHNOLOGY,125(2),173-184.
MLA	Zhang, Jiquan,et al."Cloning, expression and identification of ferritin from Chinese shrimp, Fenneropenaeus chinensis".JOURNAL OF BIOTECHNOLOGY 125.2(2006):173-184.