The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis

doi:10.1016/j.fsi.2010.04.018

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	The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis
	Li, Chenghua 1; Qiu, Limei 2; Ning, Xuanxuan 3; Chen, Aiqin 1; Qin, Song 1; Wu, Huifeng 1; Zhao, Jianmin 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2010-09-01
卷号	29 期号:3 页码:530-533
关键词	Venerupis Philippinarum Tctp Tissues Expression Time-course Expression
ISSN号	1050-4648
DOI	10.1016/j.fsi.2010.04.018
文献子类	Article
英文摘要	Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.; Translationally controlled tumor protein (TCTP) is one of the abundant and ubiquitously expressed proteins in metazoans In the present study, the first molluscan TCTP (denoted as VpTCTP) was identified from Venerupis philippinarum haemocytes by EST and RACE approaches The full-length cDNA of VpTCTP consisted of 1148 nucleotides with an open-reading frame of 555 bp encoding 184 amino acids The deduced amino acid sequence of VpTCTP shared high similarity with TCTPs from other species, indicating that VpTCTP should be a new member of TCTP family Several highly conserved motifs, including 5'terminal ologopyrimidine (5'TOP) starting sequence and rich AU and AUUT elements in 3'UTR, were also identified in VpTCTP The tissue and temporal expression of VpTCTP after Vi boo anguillarum challenge was recorded by quantitative real-time RT-PCR. VpTCTP transcript could be detected in all examined tissues with the highest expression level in haemocytes and the lowest in hepatopancreas Concerning the time-course expression in haemocytes, the relative expression of VpTCTP mRNA was down-regulated sharply from 6 h to 12 h post-infection. Then, the expression level was obviously up-regulated and reached 3.4-fold to that in the control group at 48 h post challenge As time progressed, the expression of VpTCTP recovered to the original level at 96 h. All these results indicated that VpTCTP was an acute-phase protein involved in the Immune response of V philippinarum (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000280513700020
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5943]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.SOA, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Li, Chenghua,Qiu, Limei,Ning, Xuanxuan,et al. The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis[J]. FISH & SHELLFISH IMMUNOLOGY,2010,29(3):530-533.
APA	Li, Chenghua.,Qiu, Limei.,Ning, Xuanxuan.,Chen, Aiqin.,Qin, Song.,...&Zhao, Jianmin.(2010).The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis.FISH & SHELLFISH IMMUNOLOGY,29(3),530-533.
MLA	Li, Chenghua,et al."The first molluscan TCTP in Venerupis philippinarum: Molecular cloning and expression analysis".FISH & SHELLFISH IMMUNOLOGY 29.3(2010):530-533.