Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge

doi:10.1016/j.ijhydene.2008.07.008

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	Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge
	Zhu, Daling 1; Wang, Guangce 1,2; Qiao, Honjin 2; Cai, Jinling 2
刊名	INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
	2008-11-01
卷号	33 期号:21 页码:6116-6123
关键词	Mangrove Sludge Pantoea Agglomerans Fermentation Hydrogen Production
ISSN号	0360-3199
DOI	10.1016/j.ijhydene.2008.07.008
文献子类	Article
英文摘要	A new fermentative hydrogen-producing bacterium was isolated from mangrove sludge and identified as Pantoea agglomerans using light microscopic examination, Biolog test and 16S rRNA gene sequence analysis. The isolated bacterium, designated as P. agglomerans BH-18, is a new strain that has never been optimized as a potential hydrogen-producing bacterium. In this study, the culture conditions and the hydrogen-producing ability of P. agglomerans BH-18 were examined. The strain was a salt-tolerant facultative anaerobe with the initial optimum pH value at 8.0-9.0 and temperature at 30 degrees C on cell growth. During fermentation, hydrogen started to evolve when cell growth entered late-exponential phase and was mainly produced in the stationary phase. The strain was able to produce hydrogen over a wide range of initial pH from 5 to 10, with an optimum initial pH of 6. The level of hydrogen production was affected by the initial glucose concentration, and the optimum value was found to be 10 g glucose/l. The maximum hydrogen-producing yield (2246 ml/l) and overall hydrogen production rate (160 ml/l/h) were obtained at an initial glucose concentration of 10 g/l and an initial pH value of 7.2 in marine culture conditions. In particular, the level of hydrogen production was also affected by the salt concentration. Hydrogen production reached a higher level in fresh culture conditions than in marine ones. In marine conditions, hydrogen productivity was 108 ml/l/h at an initial glucose concentration of 20 g/l and pH value of 7.2, whereas, it increased by 27% in fresh conditions. In addition, this strain could produce hydrogen using glucose and many other carbon sources such as fructose, sucrose, sorbitol and so on. As a result, it is possible that P. agglomerans BH-18 is used for biohydrogen production and biological treatment of mariculture wastewater and marine organic waste. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.; A new fermentative hydrogen-producing bacterium was isolated from mangrove sludge and identified as Pantoea agglomerans using light microscopic examination, Biolog test and 16S rRNA gene sequence analysis. The isolated bacterium, designated as P. agglomerans BH-18, is a new strain that has never been optimized as a potential hydrogen-producing bacterium. In this study, the culture conditions and the hydrogen-producing ability of P. agglomerans BH-18 were examined. The strain was a salt-tolerant facultative anaerobe with the initial optimum pH value at 8.0-9.0 and temperature at 30 degrees C on cell growth. During fermentation, hydrogen started to evolve when cell growth entered late-exponential phase and was mainly produced in the stationary phase. The strain was able to produce hydrogen over a wide range of initial pH from 5 to 10, with an optimum initial pH of 6. The level of hydrogen production was affected by the initial glucose concentration, and the optimum value was found to be 10 g glucose/l. The maximum hydrogen-producing yield (2246 ml/l) and overall hydrogen production rate (160 ml/l/h) were obtained at an initial glucose concentration of 10 g/l and an initial pH value of 7.2 in marine culture conditions. In particular, the level of hydrogen production was also affected by the salt concentration. Hydrogen production reached a higher level in fresh culture conditions than in marine ones. In marine conditions, hydrogen productivity was 108 ml/l/h at an initial glucose concentration of 20 g/l and pH value of 7.2, whereas, it increased by 27% in fresh conditions. In addition, this strain could produce hydrogen using glucose and many other carbon sources such as fructose, sucrose, sorbitol and so on. As a result, it is possible that P. agglomerans BH-18 is used for biohydrogen production and biological treatment of mariculture wastewater and marine organic waste. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.
学科主题	Chemistry, Physical ; Energy & Fuels ; Environmental Sciences ; Physics, Atomic, Molecular & Chemical
URL标识	查看原文
语种	英语
WOS记录号	WOS:000261115300029
公开日期	2010-12-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/5775]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Daling,Wang, Guangce,Qiao, Honjin,et al. Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2008,33(21):6116-6123.
APA	Zhu, Daling,Wang, Guangce,Qiao, Honjin,&Cai, Jinling.(2008).Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,33(21),6116-6123.
MLA	Zhu, Daling,et al."Fermentative hydrogen production by the new marine Pantoea agglomerans isolated from the mangrove sludge".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 33.21(2008):6116-6123.