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	A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones
	Li, DP; Zhou, ZG; Liu, HH; Wu, CY
刊名	HYDROBIOLOGIA
	1999-04-01
卷号	399页码:473-476
关键词	Laminaria Japonica Gametophyte Clones Crossing Strain Selection Sporeling Raising
ISSN号	0018-8158
文献子类	Article
英文摘要	One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.; One-celled female and male gametophytes of three Laminaria japonica strains were isolated, cultured and gametophyte clones were formed. A technique combining strain selection with sporeling raising by the use of these female and male gametophyte clones was studied. Experiments on 9 different crossing combinations was conducted in November of 1997 in Qingdao, P. R. China. The main economic characteristics, frond length and fresh weight, of sporophytes of different crossing combinations were measured. F-1 sporophytes of No. 2 showed a higher fresh weight and longer length, therefore, No. 2 (Wh860 + x Lid) was selected as a good combination. Its parental female and male gametophyte clones are being mass cultured for sporeling production. By this method, the time needed for strain selection was shortened from 5-6 to 2 years. As compared with the routine method of sporeling raising by the collection of zoospores, the time of sporeling raising of this method decrease by 50%, and the production cost is also reduced by 50%. It is believed that this method will be labour and time saving and a more economic way for strain selection and sporeling raising in L. japonica cultivation industry.
学科主题	Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000082676900059
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3355]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, DP,Zhou, ZG,Liu, HH,et al. A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones[J]. HYDROBIOLOGIA,1999,399:473-476.
APA	Li, DP,Zhou, ZG,Liu, HH,&Wu, CY.(1999).A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones.HYDROBIOLOGIA,399,473-476.
MLA	Li, DP,et al."A new method of Laminaria japonica strain selection and sporeling raising by the use of gametophyte clones".HYDROBIOLOGIA 399(1999):473-476.

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