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	Isolation of PSI from a siphonous marine green alga, Codium fragile
	Chen, M; Li, AF; Zhou, BC
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2001-12-01
卷号	28期号:6页码:858-861
关键词	Psi Cci Sucrose Gradient Centrifugation Peptide Codium Fragile
ISSN号	1000-3282
文献子类	Article
英文摘要	Three different forms of PS I complexes were isolated from a siphonous marine green alga, Codium fragile, by Triton X-100 sucrose gradient centrifugation. Zone III had a Chl a/b>20, and designated as PS I. core complex CC I because it created only CP I band in mild PAGE. Zone IV and V had absorption at 436 and 674 nm, 467 and 650 nm, and 540 nm, suggesting the presence of Chl a, Chl b, siphonaxanthin and siphonein, Chl a/b were 3.23 and 2.4, respectively. Both CP I and CP I a bands were observed when they were subjected to mild PAGE. Therefore, Zone IV and V were different forms of PS I complexes that consisted of CC I and different amount of light-harvesting complex LHC I. Zone III contained only 66 and 56 ku peptides in SDS-PAGE, while Zone IV and V had 4 different LHC I peptides of 25, 26, 26.2 and 27.5 ku in addition to 66, 56 ku peptides. Fluorescence emission spectra showed that efficient energy transfer were kept among pigments in isolated PS I complexes. Excitation energy absorbed by Chl b, siphonaxanthin and siphonein can be transferred to Chl a.; Three different forms of PS I complexes were isolated from a siphonous marine green alga, Codium fragile, by Triton X-100 sucrose gradient centrifugation. Zone III had a Chl a/b>20, and designated as PS I. core complex CC I because it created only CP I band in mild PAGE. Zone IV and V had absorption at 436 and 674 nm, 467 and 650 nm, and 540 nm, suggesting the presence of Chl a, Chl b, siphonaxanthin and siphonein, Chl a/b were 3.23 and 2.4, respectively. Both CP I and CP I a bands were observed when they were subjected to mild PAGE. Therefore, Zone IV and V were different forms of PS I complexes that consisted of CC I and different amount of light-harvesting complex LHC I. Zone III contained only 66 and 56 ku peptides in SDS-PAGE, while Zone IV and V had 4 different LHC I peptides of 25, 26, 26.2 and 27.5 ku in addition to 66, 56 ku peptides. Fluorescence emission spectra showed that efficient energy transfer were kept among pigments in isolated PS I complexes. Excitation energy absorbed by Chl b, siphonaxanthin and siphonein can be transferred to Chl a.
学科主题	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000172947500021
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3309]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Yantai Univ, Dept Biochem, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Chen, M,Li, AF,Zhou, BC. Isolation of PSI from a siphonous marine green alga, Codium fragile[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2001,28(6):858-861.
APA	Chen, M,Li, AF,&Zhou, BC.(2001).Isolation of PSI from a siphonous marine green alga, Codium fragile.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,28(6),858-861.
MLA	Chen, M,et al."Isolation of PSI from a siphonous marine green alga, Codium fragile".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 28.6(2001):858-861.

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