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	Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis
	Sun, X; Yang, GP; Mao, YX; Zhang, XC; Sui, ZH; Qin, S
刊名	PROGRESS IN NATURAL SCIENCE
	2002-07-01
卷号	12期号:7页码:518-523
关键词	Gracilaria Lemaneiformis Expressed Sequence Tags (Est) Homologous Sequence
ISSN号	1002-0071
文献子类	Article
英文摘要	The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively.; The expressed sequence tags (EST) has been proved to be a useful tool for discovering and identifying functional genes, especially in some species whose genetic information is unavailable. A total of 180 ESTs have been generated from a cDNA library of gametophytic Gracilaria lemaneiformis in this study. These clones are clustered into 151 groups, among which 8 groups are highly homologous to chloroplast genes and are abundant in the library. After searching for matches in the EST database of red alga, 22 groups are found to match with the registered ESTs of Rhadophyta and 6 with Gracilaria. Searching in the protein database reveal that 73 non-redundant clones have significant similarity to some known sequences, the majority of which are involved in photosynthesis, DNA transcription or translation, and 6, 4 and 3 clones are associated with growth or development, signal transduction and stress or defense response, respectively.
学科主题	Multidisciplinary Sciences
语种	英语
WOS记录号	WOS:000176691600006
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3287]
专题	海洋研究所_实验海洋生物学重点实验室 海洋研究所_海洋生态与环境科学重点实验室
作者单位	1.Ocean Univ Qingdao, Coll Marine Life Sci, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, X,Yang, GP,Mao, YX,et al. Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis[J]. PROGRESS IN NATURAL SCIENCE,2002,12(7):518-523.
APA	Sun, X,Yang, GP,Mao, YX,Zhang, XC,Sui, ZH,&Qin, S.(2002).Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis.PROGRESS IN NATURAL SCIENCE,12(7),518-523.
MLA	Sun, X,et al."Analysis of expressed sequence tags of a marine red alga, Gracilaria lemaneiformis".PROGRESS IN NATURAL SCIENCE 12.7(2002):518-523.

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