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	Study on the GABA gated channels in the neurosecretory cells of MTXO in the eyestalks of Eriocheir sinensis
	Sun, JS; Gao, CL; Xiang, JH
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2003-02-01
卷号	30期号:1页码:129-133
关键词	Eriocheir Sinensis Mtxo Neurosecretory Cells Gamma-aminobutyric Acid (Gaba) Whole-cell Patch Clamp Technique
ISSN号	1000-3282
文献子类	Article
英文摘要	The responses to rapid application of gamma-aminobutyric acid (GABA) and the GABA receptor characteristics of MTXO neurosecretory cells in the eyestalks of Chinese mitten-handed crab (Eriocheir sinensis) were examined by whole-cell patch clamp. Under current clamp mode, the depolarization and hyperpolarization were evoked from the three types of neurosecretory cells in response to the GABA (0.1 mmol/L) depending on the Nernst Cl(-) potential. Under voltage clamp mode, the inward Cl(-) channel currents (I(GABA)) were resolved from all three types of neurosecretory cells in response to GABA (0.01similar to5 mmol/L). The GABA currents were activated within 1 200 ms and peaked within 800 ms. No obviously desensitization was observed during GABA application. The dose-response curve showed usual S-shape, with a just-discernible effect at 0.01 mmol/L and near-saturation at 0.5 mmol/L. The GABA currents had reversal potentials that followed Nernst Cl(-) potentials when [Cl(-)] was varied. The pharmacological results revealed that the GABA receptor of the crab neurosecretory cells was sensitive to the Cl(-) channel blockers picrotoxin and niflumic acid (0.5 mmol/L), insensitive to GABA(A) receptor antagonist bicuculline and GABA(C) receptor agonist cis-4-aminocrotonic acid (CACA 1 mmol/L) and trans-4-aminocrotonic (TACA 1 mmol/L).; The responses to rapid application of gamma-aminobutyric acid (GABA) and the GABA receptor characteristics of MTXO neurosecretory cells in the eyestalks of Chinese mitten-handed crab (Eriocheir sinensis) were examined by whole-cell patch clamp. Under current clamp mode, the depolarization and hyperpolarization were evoked from the three types of neurosecretory cells in response to the GABA (0.1 mmol/L) depending on the Nernst Cl- potential. Under voltage clamp mode, the inward Cl- channel currents (I-GABA) were resolved from all three types of neurosecretory cells in response to GABA (0.01similar to5 mmol/L). The GABA currents were activated within 1 200 ms and peaked within 800 ms. No obviously desensitization was observed during GABA application. The dose-response curve showed usual S-shape, with a just-discernible effect at 0.01 mmol/L and near-saturation at 0.5 mmol/L. The GABA currents had reversal potentials that followed Nernst Cl- potentials when [Cl-] was varied. The pharmacological results revealed that the GABA receptor of the crab neurosecretory cells was sensitive to the Cl- channel blockers picrotoxin and niflumic acid (0.5 mmol/L), insensitive to GABA(A) receptor antagonist bicuculline and GABA(C) receptor agonist cis-4-aminocrotonic acid (CACA 1 mmol/L) and trans-4-aminocrotonic (TACA 1 mmol/L).
学科主题	Biochemistry & Molecular Biology ; Biophysics
语种	英语
WOS记录号	WOS:000181216300024
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3263]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Normal Univ, Coll Chem Life Sci, Tianjin 300074, Peoples R China
推荐引用方式 GB/T 7714	Sun, JS,Gao, CL,Xiang, JH. Study on the GABA gated channels in the neurosecretory cells of MTXO in the eyestalks of Eriocheir sinensis[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(1):129-133.
APA	Sun, JS,Gao, CL,&Xiang, JH.(2003).Study on the GABA gated channels in the neurosecretory cells of MTXO in the eyestalks of Eriocheir sinensis.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(1),129-133.
MLA	Sun, JS,et al."Study on the GABA gated channels in the neurosecretory cells of MTXO in the eyestalks of Eriocheir sinensis".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.1(2003):129-133.

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