Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis
	Wang, XL; Liu, CL ; Li, XJ ; Cong, YZ ; Duan, DL
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2005-06-01
卷号	47 期号:6 页码:753-758
关键词	Gametophytes Genetic Analysis Inter-simple Sequence Repeat Laminaria Sequence Characterized Amplified Region
ISSN号	1672-9072
文献子类	Article
英文摘要	Inter-simple sequence repeat (ISSR) analysis was used to assess genetic diversity among 10 pairs of male and female Laminaria gametophytes. A total of 58 amplification loci was obtained from 10 selected ISSR primers, of which 34 revealed polymorphism among the gametophytes. Genetic distances were calculated with the Dice coefficient ranging from 0.006 to 0.223. A dendrogram based on the unweighted pair-group method arithmetic (UPGMA) average showed that most male and female gametophytes of the same species were clustered together and that 10 pairs of gametophytes were divided into four groups. This was generally consistent with the taxonomic categories. The main group consisted of six pairs of gametophytes, which were selected from Laminaria japonica Aresch. by intensive inbreeding through artificial hybridization. One specific marker was cloned, but was not converted successfully into a sequence characterized amplified region (SCAR) marker. Our results demonstrate the feasibility of applying ISSR markers to evaluate Laminaria germplasm diversities.; Inter-simple sequence repeat (ISSR) analysis was used to assess genetic diversity among 10 pairs of male and female Laminaria gametophytes. A total of 58 amplification loci was obtained from 10 selected ISSR primers, of which 34 revealed polymorphism among the gametophytes. Genetic distances were calculated with the Dice coefficient ranging from 0.006 to 0.223. A dendrogram based on the unweighted pair-group method arithmetic (UPGMA) average showed that most male and female gametophytes of the same species were clustered together and that 10 pairs of gametophytes were divided into four groups. This was generally consistent with the taxonomic categories. The main group consisted of six pairs of gametophytes, which were selected from Laminaria japonica Aresch. by intensive inbreeding through artificial hybridization. One specific marker was cloned, but was not converted successfully into a sequence characterized amplified region (SCAR) marker. Our results demonstrate the feasibility of applying ISSR markers to evaluate Laminaria germplasm diversities.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
WOS记录号	WOS:000229518500014
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/3137]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.State Ocean Adm, Inst Oceanog 1, Qingdao 266061, Peoples R China 4.Shandong Oriental Ocean Sci Tech Co Ltd, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wang, XL,Liu, CL,Li, XJ,et al. Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2005,47(6):753-758.
APA	Wang, XL,Liu, CL,Li, XJ,Cong, YZ,&Duan, DL.(2005).Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,47(6),753-758.
MLA	Wang, XL,et al."Assessment of genetic diversities of selected Laminaria (Laminariales, Phaeophyta) gametophytes by inter-simple sequence repeat analysis".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 47.6(2005):753-758.