Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties | |
Sun, Baojian1; Robertsen, Borre1; Wang, Zhiqiang2,3; Liu, Bin2,3 | |
刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY |
2009 | |
卷号 | 33期号:4页码:547-558 |
关键词 | Interferon Promoter Antiviral Fish Reptile Anolis Dsrna Virus Evolution |
ISSN号 | 0145-305X |
DOI | 10.1016/j.dci.2008.10.001 |
文献子类 | Article |
英文摘要 | A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved.; A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone I gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22-32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or To cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-beta through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kappa B. In contrast, S-27609 induces mammalian IFN-alpha in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-kappa B. The presence of an NF-kappa B site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-beta. In contrast, the apparent lack of NF-kappa B motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-alpha. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-beta and IFN-alpha, respectively, but appear to utilize similar induction pathways. (C) 2008 Elsevier Ltd. All rights reserved. |
学科主题 | Immunology ; Zoology |
URL标识 | 查看原文 |
语种 | 英语 |
WOS记录号 | WOS:000263781100016 |
公开日期 | 2010-12-22 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/3017] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
作者单位 | 1.Univ Tromso, Norwegian Coll Fishery Sci, Dept Marine Biotechnol, N-9037 Tromso, Norway 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,et al. Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2009,33(4):547-558. |
APA | Sun, Baojian,Robertsen, Borre,Wang, Zhiqiang,&Liu, Bin.(2009).Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,33(4),547-558. |
MLA | Sun, Baojian,et al."Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 33.4(2009):547-558. |
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