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	The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)
	Li, Fuchao; Qin, Song; Jiang, Peng; Wu, Yun; Zhang, Wei
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2009-06-01
卷号	21期号:3页码:287-293
关键词	Fcp Gus Integrative Expression Laminaria Japonica Promoter
ISSN号	0921-8971
DOI	10.1007/s10811-008-9366-9
文献子类	Article
英文摘要	In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.; In this study, the background activity of beta-glucuronidase (GUS) was analyzed histochemically and fluorometrically in the negative control of Laminaria japonica (Phaeophyta) thalli, showing low level of activity. GUS gene transformation without selectable gene in L. japonica was performed using four different promoters, i.e., Cauliflower mosaic virus 35S promoter (CaMV35S) from cauliflower mosaic virus, ubiquitin promoter (UBI) from maize, adenine-methyl transfer enzyme gene promoter (AMT) from virus in green alga Chlorella, and fucoxanthin chlorophyll a/c-binding protein gene promoter (FCP) from diatom Phaeodactylum tricornutum. The GUS transient activity was determined fluorometrically after bombarding sliced parthenogenetic sporophytes explants, and it was found that the activity resulting from CaMV35S and FCP promoters (in 114.3 and 80.6 pmol MU min(-1) (mg protein)(-1), respectively) was higher than for the other two promoters. The female gametophytes were bombarded and regenerated parthenogenetic sporophytes. FCP was the only promoter that resulted in detectable GUS chimeric expression activity during histochemical staining and polymerase chain reaction. Results of Southern blot showed that GUS gene was integrated with the L. japonica genome.
学科主题	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000265498600004
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2965]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li, Fuchao,Qin, Song,Jiang, Peng,et al. The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)[J]. JOURNAL OF APPLIED PHYCOLOGY,2009,21(3):287-293.
APA	Li, Fuchao,Qin, Song,Jiang, Peng,Wu, Yun,&Zhang, Wei.(2009).The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta).JOURNAL OF APPLIED PHYCOLOGY,21(3),287-293.
MLA	Li, Fuchao,et al."The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)".JOURNAL OF APPLIED PHYCOLOGY 21.3(2009):287-293.

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