Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis

doi:10.1007/s00343-009-9168-1

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	Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis
	Li Yanyan 1; Shen Songdong 1; He Lihong 1; Xu Pu 2; Wang Guangce 3
刊名	CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2009-08-01
卷号	27 期号:3 页码:493-501
关键词	Porphyra Haitanensis Its Region Sequence Analysis Interspecies Phylogeny
ISSN号	0254-4059
DOI	10.1007/s00343-009-9168-1
文献子类	Article
英文摘要	The sequences of the ITS (internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli (NB, PT and ST) were amplified, sequenced and analyzed. In addition, the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated. The results are as follows: the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains. The sequences of ITS l were 331 by to 334 bp, while those of the 5.8S rDNA were 158 by and the sequences of ITS2 ranged from 673 by to 681 bp. The sequences of the ITS had a high level of homology (up to 99.5%) with that of P. haitanensis (DQ662228) retrieved from GenBank, but were only approximately 50% homologous with those of other species of Porphyra. The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis. These results suggest that the three cultivated strains of P. haitanensis evolved conservatively and that the ITS showed evolutionary consistency. However, the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations. Therefore, the relationship of Porphyra interspecies phyletic evolution could be judged, which provides the proof for Porphyra identification study. However, proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships.; The sequences of the ITS (internal transcribed spacer) and 5.8S rDNA of three cultivated strains of Porphyra haitanensis thalli (NB, PT and ST) were amplified, sequenced and analyzed. In addition, the phylogenic relationships of the sequences identified in this study with those of other Porphyra retrieved from GenBank were evaluated. The results are as follows: the sequences of the ITS and 5.8S rDNA were essentially identical among the three strains. The sequences of ITS l were 331 by to 334 bp, while those of the 5.8S rDNA were 158 by and the sequences of ITS2 ranged from 673 by to 681 bp. The sequences of the ITS had a high level of homology (up to 99.5%) with that of P. haitanensis (DQ662228) retrieved from GenBank, but were only approximately 50% homologous with those of other species of Porphyra. The results obtained when a phylogenetic tree was constructed coincided with the results of the homology analysis. These results suggest that the three cultivated strains of P. haitanensis evolved conservatively and that the ITS showed evolutionary consistency. However, the sequences of the ITS and 5.8S rDNA of different Porphyra species showed great variations. Therefore, the relationship of Porphyra interspecies phyletic evolution could be judged, which provides the proof for Porphyra identification study. However, proper classifications of the subspecies and the populations of Porphyra should be determined through the use of other molecular techniques to determine the genetic variability and rational phylogenetic relationships.
学科主题	Limnology ; Oceanography
URL标识	查看原文
语种	英语
WOS记录号	WOS:000270742000010
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2963]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Soochow Univ, Coll Med, Dept Cell Biol, Suzhou 215123, Peoples R China 2.Changshu Inst Technol, Dept Biol & Food Engn, Suzhou 215500, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Li Yanyan,Shen Songdong,He Lihong,et al. Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2009,27(3):493-501.
APA	Li Yanyan,Shen Songdong,He Lihong,Xu Pu,&Wang Guangce.(2009).Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,27(3),493-501.
MLA	Li Yanyan,et al."Sequence analysis of the ITS region and 5.8S rDNA of Porphyra haitanensis".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 27.3(2009):493-501.