Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts

doi:10.1134/S1021443709010166

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts
	Li, D.1,3,4; Lue, F.1,4; Wang, G.1,2; Zhou, B.4
刊名	RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
	2009
卷号	56 期号:1 页码:110-117
关键词	Bryopsis Hypnoides Assembly Cell Membrane Chloroplast Mitochondria Protoplast
ISSN号	1021-4437
DOI	10.1134/S1021443709010166
文献子类	Article
英文摘要	The chloroplasts, mitochondria, and protoplasm devoid of mature chloroplasts (PMC) of Bryopsis hypnoides Lamouroux were isolated by low-speed and sucrose density centrifugation. The PMC aggregated in artificial seawater, and then protoplasts without mature chloroplasts (PtMCs) were formed. Transmission electron microscopy and cytochemical studies indicated that there were mitochondria, nuclei, vesicles, and other small cell organelles in the PtMCs. Scanning electron microscopy showed that there were holes on the surface of 1-h PtMCs and then fewer holes on the surface of 24-h PtMCs, suggesting that a healing process occurred. The plasma membrane was formed over the surface of the PtMCs. However, the cell wall was not regenerated, and the newly formed PtMCs were ruptured and died in 3 days. Light intensity during alga maintenance before use influenced significantly (one-way ANOVA, P < 0.0001) on the number of PtMCs formed; the highest number of PtMCs was formed at 20A mu mol/(m(2) s). When isolated chloroplasts were transferred into seawater, there were only two or three chloroplasts aggregated together. However, isolated mitochondria and the mixed six layers of cell organelles (separated by sucrose density centrifugation) could not aggregate in the artificial seawater. This indicates that the conjunction of cell organelles is important for their aggregation.; The chloroplasts, mitochondria, and protoplasm devoid of mature chloroplasts (PMC) of Bryopsis hypnoides Lamouroux were isolated by low-speed and sucrose density centrifugation. The PMC aggregated in artificial seawater, and then protoplasts without mature chloroplasts (PtMCs) were formed. Transmission electron microscopy and cytochemical studies indicated that there were mitochondria, nuclei, vesicles, and other small cell organelles in the PtMCs. Scanning electron microscopy showed that there were holes on the surface of 1-h PtMCs and then fewer holes on the surface of 24-h PtMCs, suggesting that a healing process occurred. The plasma membrane was formed over the surface of the PtMCs. However, the cell wall was not regenerated, and the newly formed PtMCs were ruptured and died in 3 days. Light intensity during alga maintenance before use influenced significantly (one-way ANOVA, P < 0.0001) on the number of PtMCs formed; the highest number of PtMCs was formed at 20A mu mol/(m(2) s). When isolated chloroplasts were transferred into seawater, there were only two or three chloroplasts aggregated together. However, isolated mitochondria and the mixed six layers of cell organelles (separated by sucrose density centrifugation) could not aggregate in the artificial seawater. This indicates that the conjunction of cell organelles is important for their aggregation.
学科主题	Plant Sciences
URL标识	查看原文
语种	英语
WOS记录号	WOS:000262485000016
公开日期	2010-12-22
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/2945]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Marine Sci & Engn, Tianjin 300457, Peoples R China 3.Liaocheng Univ, Inst Agr, Dept Food Sci, Liaocheng 252059, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, D.,Lue, F.,Wang, G.,et al. Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts[J]. RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,2009,56(1):110-117.
APA	Li, D.,Lue, F.,Wang, G.,&Zhou, B..(2009).Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts.RUSSIAN JOURNAL OF PLANT PHYSIOLOGY,56(1),110-117.
MLA	Li, D.,et al."Assembly of the subcellular parts of Bryopsis hypnoides Lamouroux into new protoplasts".RUSSIAN JOURNAL OF PLANT PHYSIOLOGY 56.1(2009):110-117.