Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China

doi:10.1111/j.1365-2672.2007.03494.x

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	Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China
	Dang, H.; Zhang, X.; Song, L.; Chang, Y.; Yang, G.; Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China
刊名	JOURNAL OF APPLIED MICROBIOLOGY
	2007-12-01
卷号	103 期号:6 页码:2580-2592
关键词	Antibiotic Resistance Cat Gene Flor Gene Mariculture Environment Marine Pseudoalteromonas Marine Vibrios Tet Gene Vibrio Splendidus
ISSN号	1364-5072
DOI	10.1111/j.1365-2672.2007.03494.x
文献子类	Article
英文摘要	Aims: To assess the diversity of antibiotic-resistant bacteria and their resistance genes in typical maricultural environments.; Aims: To assess the diversity of antibiotic-resistant bacteria and their resistance genes in typical maricultural environments. Methods nand Results: Multidrug-resistant bacteria and resistance genes from a mariculture farm of China were analysed via cultivation and polymerase chain reaction (PCR) methods. Oxytetracycline (OTC)-resistant bacteria were abundant in both abalone and turbot rearing waters, accounting for 3.7% and 9.9% of the culturable microbes. Multidrug resistance was common, with simultaneous resistance to OTC, chloramphenicol and ampicillin the most common resistance phenotype. 16S rDNA sequence analyses indicate that the typical resistant isolates belonged to marine Vibrio, Pseudoalteromonas or Alteromonas species, with resistance most common in Vibrio splendidus isolates. For OTC resistance, tet(A), tet(B) and tet(M) genes were detected in some multidrug-resistant isolates, with tet(D) being the most common molecular determinant. For chloramphenicol resistance, cat II was common, and floR was also detected, especially in marine Pseudoalteromonas strains. Conclusions: There is the risk of multidrug-resistant bacteria contamination in mariculture environments and marine Vibrio and Pseudoalteromonas species serve as reservoirs of specific antibiotic resistance determinants. Significance and Impact of the Study: This paper and similar findings from Korea and Japan indicate the potential for widespread distribution of antibiotic resistance genes in mariculture environments from the East Asian region of the world.
学科主题	Biotechnology & Applied Microbiology ; Microbiology
URL标识	查看原文
语种	英语
WOS记录号	WOS:000251251200061
公开日期	2010-11-18
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/1590]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 2.Ocean Univ China, Life Sci & Technol Coll, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Expt Marine Biol Lab, Qingdao, Peoples R China 4.Dalian Fisheries Univ, Key Lab Mariculture & Biotechnol, Dalian, Peoples R China
推荐引用方式 GB/T 7714	Dang, H.,Zhang, X.,Song, L.,et al. Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China[J]. JOURNAL OF APPLIED MICROBIOLOGY,2007,103(6):2580-2592.
APA	Dang, H.,Zhang, X.,Song, L.,Chang, Y.,Yang, G.,&Dang, H, Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, 7 Nanhai Rd, Qingdao 266071, Peoples R China.(2007).Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China.JOURNAL OF APPLIED MICROBIOLOGY,103(6),2580-2592.
MLA	Dang, H.,et al."Molecular determination of oxytetracycline-resistant bacteria and their resistance genes from mariculture environments of China".JOURNAL OF APPLIED MICROBIOLOGY 103.6(2007):2580-2592.