Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 中国西南野生生物种质资源库

	Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4
	Ye, Jingrun 1,2; Liu, Mengmeng 1,2; He, Mingxia1 ; Ye, Ying 1,2; Huang, Junchao1
刊名	MARINE DRUGS
	2019
卷号	17 期号:1 页码:13
关键词	Aurantiochytrium Astaxanthin Docosahexaenoic acid genetic transformation
ISSN号	1660-3397
英文摘要	The marine thraustochytrids are a promising source of docosahexaenoic acid (DHA) and the ketocarotenoid astaxanthin. In this study, the biosynthetic pathways of these two important metabolites in Aurantiochytrium sp. SK4 was illustrated by the analyses of the genome, transcriptome, key enzymes, and pathway products. Two sets of genes were involved in two pathways for the biosynthesis of fatty acids. The absence of -15 desaturase genes and the presence of docosapentaenoic acid (DPA), up to 12% of total fatty acids suggest that Aurantiochytrium sp. SK4 may synthesize DHA mainly via a polyketide synthase (PKS) pathway. Three enzymes, namely geranyl diphosphate synthase (GPPS), farnysyl diphosphate synthase (FPPS), and geranylgeranyle diphosphate synthase (GGPPS) were found to be involved in the formation of GGPP that was subsequently catalyzed to -carotene by a trifunctional CrtIBY enzyme. -Carotene might be ketolated and then hydroxylated into astaxanthin based on the carotenoid profiles. The formation of GGPP was proposed to be the limiting steps for carotenoid production. Overexpression of the Archaeoglobus GPS together with the Escherichia coli isopentenyl pyrophosphate isomerase, and Vitreoscilla hemoglobin resulted in not only 1.85- and 5.02-fold increases of total carotenoids and astaxanthin, but also 2.40- and 2.74-fold increases of total fatty acids and DHA. This study provides insights into the biosynthesis of carotenoids and fatty acids in Aurantiochytrium.
语种	英语
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/64204]
专题	昆明植物研究所_中国西南野生生物种质资源库
通讯作者	Huang, Junchao
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ye, Jingrun,Liu, Mengmeng,He, Mingxia,et al. Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4[J]. MARINE DRUGS,2019,17(1):13.
APA	Ye, Jingrun,Liu, Mengmeng,He, Mingxia,Ye, Ying,&Huang, Junchao.(2019).Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4.MARINE DRUGS,17(1),13.
MLA	Ye, Jingrun,et al."Illustrating and Enhancing the Biosynthesis of Astaxanthin and Docosahexaenoic Acid in Aurantiochytrium sp. SK4".MARINE DRUGS 17.1(2019):13.