Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae

	Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae
	Li, Qiurong ; Zhang, Qiang ; Wang, Meng ; Liu, Fuzhong ; Zhao, Sumin ; Ma, Jian ; Luo, Nan ; Li, Ning ; Li, Yousheng ; Xu, Guowang
刊名	archives of biochemistry and biophysics
	2007-10-15
卷号	466 期号:2 页码:250-259
关键词	polyunsaturated fatty acids caveolae fatty acid composition phospholipids eNOS
通讯作者	黎介寿
产权排序	2;5
英文摘要	n - 3 polyunsaturated fatty acids are assumed to play an important role in the prevention and treatment of atherosclerosis. endothelial nitric-oxide synthase (enos) is responsible for cardiovascular homeostasis involving in regulation of vascular function, and the subcellular localization is critical for its activation. here we determined the effect of docosahexaenoic acid (dha, 22:6 n - 3) on distribution of enos and its activity. dha treatment markedly altered lipid environment of caveolae microdomains, which was coincided with selective displacement of caveolin-1 and enos from caveolae. akt was not detected in caveolae fractions and cam was distributed in both of caveolin-1-enriched membranes and non-caveolar fractions, whose distribution was unaffected by dha. these data demonstrated for the first time that dha altered caveolae microenvironment not only by modifying membrane lipid composition, but also by changing distribution of major structural proteins. dha-induced alterations in caveolae lipid/protein environment may be an important mechanism in the development of pathogenesis of atherosclerosis. (c) 2007 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; biophysics
研究领域[WOS]	biochemistry & molecular biology ; biophysics
关键词[WOS]	polyunsaturated fatty-acids ; protein-kinase b ; eicosapentaenoic acid ; signal-transduction ; lipid-composition ; membrane domains ; binding domain ; blood-pressure ; activation ; localization
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000250067700012
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98813]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Jinling Hosp, Inst Gen Surg, Nanjing 210002, Peoples R China 2.Chinese Acad Sci, Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China 3.Nanjing Univ, Sch Med, Nanjing 210093, Peoples R China
推荐引用方式 GB/T 7714	Li, Qiurong,Zhang, Qiang,Wang, Meng,et al. Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae[J]. archives of biochemistry and biophysics,2007,466(2):250-259.
APA	Li, Qiurong.,Zhang, Qiang.,Wang, Meng.,Liu, Fuzhong.,Zhao, Sumin.,...&Li, Jieshou.(2007).Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae.archives of biochemistry and biophysics,466(2),250-259.
MLA	Li, Qiurong,et al."Docosahexaenoic acid affects endothelial nitric oxide synthase in caveolae".archives of biochemistry and biophysics 466.2(2007):250-259.