Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets

	Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets
	Xu, Junguang ; Zhao, Xiaoming ; Wang, Xiuli ; Zhao, Zongbao ; Du, Yuguang
刊名	pesticide biochemistry and physiology
	2007-06-01
卷号	88 期号:2 页码:167-175
关键词	oligochitosan Phytophthora capsici mechanism internalization CLSM
通讯作者	杜昱光
产权排序	1;1
英文摘要	the mechanism of action of oligochitosan, which hasshown great antifungal activity against phytophthora capsici in vitro, was studied using 2-aminoacridone-labeled oligoclutosan (2-amac-oligochitosan) and a gel-retardation experiment. internalization of 2-amac-oligochitosan in cysts, gernitubes and sporangia of p. capsici was confirmed by confocal laser scan microscopy (clsm), and the degree of uptake depended on the incubation concentration. 2-amac-oligochitosan localized mainly in the cytoplasm and showed no binding to both cell wall and cell membrane. mannose, an inhibitor for oligochitosan uptake by macrophages, could not inhibit the internalization of oligochitosan in p. capsici. the gel-retardation experiment showed that oligochitosan bound strongly to dna and rna of p. capsici. these results indicate that oligochitosan exerts its antifungal activity by penetrating the cell membrane and putative binding to intracellular targets such as dna and rna. (c) 2006 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; entomology ; physiology
研究领域[WOS]	biochemistry & molecular biology ; entomology ; physiology
关键词[WOS]	antimicrobial peptide ; chitosan ; chitooligosaccharides ; receptor ; pea
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000246930700008
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/98573]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xu, Junguang,Zhao, Xiaoming,Wang, Xiuli,et al. Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets[J]. pesticide biochemistry and physiology,2007,88(2):167-175.
APA	Xu, Junguang,Zhao, Xiaoming,Wang, Xiuli,Zhao, Zongbao,&Du, Yuguang.(2007).Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets.pesticide biochemistry and physiology,88(2),167-175.
MLA	Xu, Junguang,et al."Oligochitosan inhibits Phytophthora capsici by penetrating the cell membrane and putative binding to intracellular targets".pesticide biochemistry and physiology 88.2(2007):167-175.