Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy | |
Wang, Xuetao3; Gan, Qinhua2,6; Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China); Xie, Zhenyu6; Wang, Yun1; Lu, Yandu5,6; Tian, Yang4 | |
刊名 | ADVANCED SCIENCE |
2017-11-01 | |
卷号 | 4期号:11页码:1 |
关键词 | Crop Pathogens Culture-free Detection Micro-raman Spectroscopy Single Cells |
DOI | 10.1002/advs.201700127 |
文献子类 | Article |
英文摘要 | The rapid and sensitive identification of invasive plant pathogens has important applications in biotechnology, plant quarantine, and food security. Current methods are far too time-consuming and need a pre-enrichment period ranging from hours to days. Here, a micro-Raman spectroscopy-based bioassay for culture-free pathogen quarantine inspection at the single cell level within 40 min is presented. The application of this approach can readily and specifically detect plant pathogens Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi that are closely related pathogenically. Furthermore, the single-bacterium detection was able to discriminate them from a reference Raman spectral library including multiple quarantine-relevant pathogens with broad host ranges and an array of pathogenic variants. To show the usefulness of this assay, Burkholderia gladioli pv. alliicola and Erwinia chrysanthemi are detected at single-bacterium level in plant tissue lesions without pre-enrichment. The results are confirmed by the plate-counting method and a genetic molecular approach, which display comparable recognition ratios to the Raman spectroscopy-based bioassay. The results represent a critical step toward the use of micro-Raman spectroscopy in rapid and culture-free discrimination of quarantine relevant plant pathogens. |
URL标识 | 查看原文 |
WOS关键词 | Rapid Identification ; Bacteria ; Microspectroscopy ; Microorganisms ; Capture ; Threat ; Blood ; Spp. |
语种 | 英语 |
WOS记录号 | WOS:000416155900004 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://ir.idsse.ac.cn/handle/183446/5267] |
专题 | 文献信息中心 |
通讯作者 | Lu, Yandu(Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China); Lu, Yandu |
作者单位 | 1.Shanghai Hesen Biotech Co LTD, Shanghai 201802, Peoples R China 2.Shandong Entry Exit Inspect & Quarantine Bur, Inspect & Quarantine Technol Ctr, Qingdao 266002, Shandong, Peoples R China 3.Hisense Co, Qingdao 266555, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 5.Hainan Univ, Minist Educ, Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China 6.Hainan Univ, Coll Oceanol, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xuetao,Gan, Qinhua,Lu, Yandu,et al. Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy[J]. ADVANCED SCIENCE,2017,4(11):1. |
APA | Wang, Xuetao.,Gan, Qinhua.,Lu, Yandu.,Xie, Zhenyu.,Wang, Yun.,...&Tian, Yang.(2017).Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy.ADVANCED SCIENCE,4(11),1. |
MLA | Wang, Xuetao,et al."Culture-Free Detection of Crop Pathogens at the Single-Cell Level by Micro-Raman Spectroscopy".ADVANCED SCIENCE 4.11(2017):1. |
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