De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids

doi:10.1016/j.aquaculture.2018.01.012

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids
	Zhang, Haibin; Chen, Jiawei; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China); Liu, Helu; Kong, Xue; Haibin Zhang
刊名	AQUACULTURE
	2018-04-01
卷号	491 页码:358-367
关键词	Sea Cucumber Triterpene Glycosides Transcriptome Biosynthesis Cyclase
DOI	10.1016/j.aquaculture.2018.01.012
产权排序	第1完成单位
文献子类	Article
英文摘要	As the widespread species in the tropical Indo-Pacific area, the sea cucumber Stichopus horrens is characterized by the presence of appreciable amounts of triterpene glycosides. However, genes involved in biosynthesis of the triterpenoids in sea cucumber have rarely been researched. Here, we aim to explore the triterpenoid biosynthesis of S. horrens collected in the south sea of China. In this study, the de novo sequencing of S. horrens was carried out by Illumina HiSeq 2000. A total of 99,854,932 high quality sequence reads were obtained. These raw data were assembled into 168,151 contigs, and 78,024 unigenes were left after redundance removing. The transcriptomic data clearly showed that triterpenoid biosynthesis was via the MVA pathway in S. horrens. The content of total triterpene glycosides was examined by method of orcinol reaction, and 0.116 +/- 0.012 mg/g and 0.016 +/- 0.003 mg/g in intestine and body wall, respectively. qRTPCR showed that both oxidosqualene cyclases OSC1 and OSC2 are highly expressed in intestine, suggesting their commitment to triterpenoid biosynthesis. We also reported some potential genes encoding Cytochrome P450 and glycosylations enzymes, which might be involved in the late step of biosynthesis of triterpenoid glycosides. Overall, the present study provided the basic data for future molecular studies on biosynthesis of triterpene glycosides in sea cucumber.
学科主题	生物化学 ; 细胞生物学 ; 分子生物学
URL标识	查看原文
WOS关键词	SPONGE ECTYOPLASIA-FEROX ; SEA-CUCUMBERS ; BIOLOGICAL-ACTIVITIES ; MARINE-INVERTEBRATES ; GLYCOSIDES ; SAPONINS ; ECHINODERMATA ; STARFISH ; GINSENG
语种	英语
WOS记录号	WOS:000431009400046
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.idsse.ac.cn/handle/183446/5942]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin; Zhang, Haibin(Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China); Haibin Zhang
作者单位	Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Haibin,Chen, Jiawei,Zhang, Haibin,et al. De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids[J]. AQUACULTURE,2018,491:358-367.
APA	Zhang, Haibin,Chen, Jiawei,Zhang, Haibin,Liu, Helu,Kong, Xue,&Haibin Zhang.(2018).De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids.AQUACULTURE,491,358-367.
MLA	Zhang, Haibin,et al."De novo sequencing and transcriptome analysis of Stichopus horrens to reveal genes related to biosynthesis of triterpenoids".AQUACULTURE 491(2018):358-367.