siRNA transfection in larvae of the barnacle Amphibalanus amphitrite

doi:10.1242/jeb.120113

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	siRNA transfection in larvae of the barnacle Amphibalanus amphitrite
	Wong, Yue Him 2; Qian, Pei-Yuan 1,2; Yu, Li 1,2; Zhang, Gen 1,2; He, Li-Sheng2,3
刊名	JOURNAL OF EXPERIMENTAL BIOLOGY
	2015-08-01
卷号	218 期号:16 页码:2505-2509
关键词	Transfection P38 Mapk Rna Interference Amphibalanus Amphitrite Larval Settlement
DOI	10.1242/jeb.120113
文献子类	Article
英文摘要	RNA interference (RNAi) provides an efficient and specific technique for functional genomic studies. Yet, no successful application of RNAi has been reported in barnacles. In this study, siRNA against p38 MAPK was synthesized and then transfected into A. amphitrite larvae at either the nauplius or cyprid stage, or at both stages. Effects of siRNA transfection on the p38 MAPK level were hardly detectable in the cyprids when they were transfected at the nauplius stage. In contrast, larvae that were transfected at the cyprid stage showed lower levels of p38 MAPK than the blank and reagent controls. However, significantly decreased levels of phosphorylated p38 MAPK (pp38 MAPK) and reduced settlement rates were observed only in 'double transfections', in which larvae were exposed to siRNA solution at both the nauplius and cyprid stages. A relatively longer transfection time and more larval cells directly exposed to siRNA might explain the higher efficiency of double transfection experiments.
WOS关键词	DISTAL-LESS ; RNA INTERFERENCE ; GENE ; EXPRESSION ; MODULATION ; KNOCKDOWN ; SYSTEM ; DSRNA ; PCR
语种	英语
WOS记录号	WOS:000359937300007
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/1213]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
作者单位	1.Hong Kong Univ Sci & Technol, Sch Sci, Environm Sci Programs, Kowloon, Hong Kong, Peoples R China 2.Hong Kong Univ Sci & Technol, Sch Sci, Div Life Sci, KAUST Global Collaborat Res Program, Kowloon, Hong Kong, Peoples R China 3.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya City 572000, Hainan Province, Peoples R China
推荐引用方式 GB/T 7714	Wong, Yue Him,Qian, Pei-Yuan,Yu, Li,et al. siRNA transfection in larvae of the barnacle Amphibalanus amphitrite[J]. JOURNAL OF EXPERIMENTAL BIOLOGY,2015,218(16):2505-2509.
APA	Wong, Yue Him,Qian, Pei-Yuan,Yu, Li,Zhang, Gen,&He, Li-Sheng.(2015).siRNA transfection in larvae of the barnacle Amphibalanus amphitrite.JOURNAL OF EXPERIMENTAL BIOLOGY,218(16),2505-2509.
MLA	Wong, Yue Him,et al."siRNA transfection in larvae of the barnacle Amphibalanus amphitrite".JOURNAL OF EXPERIMENTAL BIOLOGY 218.16(2015):2505-2509.