High-throughput transcriptome sequencing of the cold seep mussel Bathymodiolus platifrons

doi:10.1038/srep16597

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海生物蛋白质组学及分子生物学研究组

	High-throughput transcriptome sequencing of the cold seep mussel Bathymodiolus platifrons
	Sun, Jin 1; Wong, Yue Him 3; Qian, Pei-Yuan 3; Qiu, Jian-Wen 1; Chen, Lian Guo 3; He, Li Sheng2
刊名	SCIENTIFIC REPORTS
	2015-11-23
卷号	5
DOI	10.1038/srep16597
文献子类	Article
英文摘要	Bathymodiolid mussels dominate hydrothermal vents, cold methane/sulfide-hydrocarbon seeps, and other sites of organic enrichment. Here, we aimed to explore the innate immune system and detoxification mechanism of the deep sea mussel Bathymodiolus platifrons collected from a methane seep in the South China Sea. We sequenced the transcriptome of the mussels' gill, foot and mantle tissues and generated a transcriptomic database containing 96,683 transcript sequences. Based on GO and KEGG annotations, we reported transcripts that were related to the innate immune system, heavy metal detoxification and sulfide metabolic genes. Our in-depth analysis on the isoforms of peptidoglycan recognition protein (PGRP) that have different cellular location and potentially differential selectivity towards peptidoglycan (PGN) from gram-positive and gram-negative bacteria were differentially expressed in different tissues. We also reported a potentially novel form of metallothionein and the production of phytochelatin in B. platifrons, which has not been reported in any of its coastal relative Mytilus mussel species. Overall, the present study provided new insights into heavy metal and sulfide metabolism in B. platifrons and can be served as the basis for future molecular studies on host-symbiont interactions in cold seep mussels.
WOS关键词	PEPTIDOGLYCAN RECOGNITION PROTEIN ; HEAVY-METAL DETOXIFICATION ; OUTER-MEMBRANE PROTEIN ; DEEP-SEA MUSSELS ; HYDROTHERMAL VENTS ; TAURINE TRANSPORTER ; METHANE SEEP ; RNA-SEQ ; THIOTROPHIC ENDOSYMBIONTS ; PHYTOCHELATIN SYNTHASE
语种	英语
WOS记录号	WOS:000365085100001
内容类型	期刊论文
源URL	[http://124.16.218.2:8080/handle/183446/893]
专题	深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组
作者单位	1.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Hainan, Peoples R China 3.Hong Kong Univ Sci & Technol, Sch Sci, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jin,Wong, Yue Him,Qian, Pei-Yuan,et al. High-throughput transcriptome sequencing of the cold seep mussel Bathymodiolus platifrons[J]. SCIENTIFIC REPORTS,2015,5.
APA	Sun, Jin,Wong, Yue Him,Qian, Pei-Yuan,Qiu, Jian-Wen,Chen, Lian Guo,&He, Li Sheng.(2015).High-throughput transcriptome sequencing of the cold seep mussel Bathymodiolus platifrons.SCIENTIFIC REPORTS,5.
MLA	Sun, Jin,et al."High-throughput transcriptome sequencing of the cold seep mussel Bathymodiolus platifrons".SCIENTIFIC REPORTS 5(2015).