Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway

doi:10.1080/08927014.2015.1121245

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 深海微生物活性物质研究组

	Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway
	Han, Zhuang1,2 ; Xu, Ying 1,4; Sun, Jin 6; Zhang, Gen 5; Wang, Kai-Ling 1,3,4; Qian, Pei-Yuan 1,5; Wang, Chang-Yun 3; Liu, Qing-Ai 3; Shao, Chang-Lun 3; Liu, Ling-Li 5
刊名	BIOFOULING
	2016
卷号	32 期号:1 页码:35-44
关键词	Cochliomycin a Antifouling Barnacle Nitric Oxide Cgmp Mechanism
DOI	10.1080/08927014.2015.1121245
文献子类	Article
英文摘要	Cochliomycin A is a compound with anti-barnacle settlement activity and low toxicity, but the molecular mechanism of the compound is unknown. Here, isobaric tags for the relative or absolute quantitation (iTRAQ) labeling proteomic method were applied to analyze changes in the proteome of Amphibalanus ( =Balanus) amphitrite cyprids in response to cochliomycin A treatment. Cochliomycin A affected the cytochrome P450, glutathione S-transferase (GST) and NO/cGMP pathways, among which the NO/cGMP pathway was considered to play a key role in barnacle larval settlement, while the cytochrome P450 and the GST pathways are mainly for detoxification. The results of real-time PCR further suggested the NO/cGMP pathway was activated in response to cochliomycin A. Larval settlement assays revealed that S-methylisothiourea sulfate (SMIS) and 1H-(1,2,4) oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) rescued cyprids from cochliomycin A-induced inhibition of larval settlement. The findings supported the hypothesis that cochliomycin A inhibited barnacle larval settlement by stimulating the NO/cGMP pathway.
URL标识	查看原文
WOS关键词	NITRIC-OXIDE ; ANTIFOULING COMPOUNDS ; BALANUS-AMPHITRITE ; METAMORPHOSIS ; HSP90 ; BUTENOLIDE ; MECHANISMS ; ATTACHMENT ; EXPRESSION ; SYSTEM
语种	英语
WOS记录号	WOS:000368561300005
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.idsse.ac.cn/handle/183446/1346]
专题	深海科学研究部_深海生物学研究室_深海微生物活性物质研究组
作者单位	1.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Sanya Inst Deep Sea Sci & Engn, Sanya, Peoples R China 3.Ocean Univ China, Key Lab Marine Drugs, Minist Educ China, Sch Med & Pharm, Qingdao, Peoples R China 4.Shenzhen Univ, Coll Life Sci, Shenzhen, Peoples R China 5.Hong Kong Univ Sci & Technol, Sch Sci, Environm Sci Program, Hong Kong, Hong Kong, Peoples R China 6.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Han, Zhuang,Xu, Ying,Sun, Jin,et al. Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway[J]. BIOFOULING,2016,32(1):35-44.
APA	Han, Zhuang.,Xu, Ying.,Sun, Jin.,Zhang, Gen.,Wang, Kai-Ling.,...&Liu, Ling-Li.(2016).Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway.BIOFOULING,32(1),35-44.
MLA	Han, Zhuang,et al."Cochliomycin A inhibits the larval settlement of Amphibalanus amphitrite by activating the NO/cGMP pathway".BIOFOULING 32.1(2016):35-44.