Structural insight into precursor tRNA processing by yeast ribonuclease P | |
Lan, Pengfei1; Chen, Juan1; Shi, Shaohua1; Wu, Jian1; Lei, Ming1; Tan, Ming2,3; Niu, Shuangshuang2,3,5; Cheng, Hong2; Zhang, Yuebin4; Peng, Xiangda4 | |
刊名 | SCIENCE |
2018 | |
卷号 | 362期号:6415页码:657-+ |
关键词 | Pre-transfer-rna Density-functional Thermochemistry Particle Electron Cryomicroscopy Free-energy Calculations Metal-binding Site Molecular-dynamics Crystal-structure Active-site Biological Applications 2-metal-ion Mechanism |
ISSN号 | 0036-8075 |
DOI | 10.1126/science.aat6678 |
文献子类 | Article |
英文摘要 | Ribonuclease P (RNase P) is a universal ribozyme responsible for processing the 5'-leader of pre-transfer RNA (pre-tRNA). Here, we report the 3.5-angstrom cryo-electron microscopy structures of Saccharomyces cerevisiae RNase P alone and in complex with pre-tRNA(Phe). The protein components form a hook-shaped architecture that wraps around the RNA and stabilizes RNase P into a "measuring device" with two fixed anchors that recognize the L-shaped pre-tRNA. A universally conserved uridine nucleobase and phosphate backbone in the catalytic center together with the scissile phosphate and the O3' leaving group of pre-tRNA jointly coordinate two catalytic magnesium ions. Binding of pre-tRNA induces a conformational change in the catalytic center that is required for catalysis. Moreover, simulation analysis suggests a two-metal-ion S(N)2 reaction pathway of pre-tRNA cleavage. These results not only reveal the architecture of yeast RNase P but also provide a molecular basis of how the 5'-leader of pre-tRNA is processed by eukaryotic RNase P. |
电子版国际标准刊号 | 1095-9203 |
WOS研究方向 | Multidisciplinary Sciences |
语种 | 英语 |
WOS记录号 | WOS:000450474500033 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3478] |
专题 | 生化所2018年发文 |
通讯作者 | Wu, Jian; Lei, Ming; Wang, Xuejuan |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Shanghai Inst Precis Med, Shanghai 200125, Peoples R China; 2.Chinese Acad Sci, State Key Lab Mol Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China; 4.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Lab Mol Modeling & Design, Dalian 116023, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 6.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China; 7.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China; 8.Shanghai Jiao Tong Univ, Sch Med, Chinese Minist Educ, Key Lab Cell Differentiat & Apoptosis, Shanghai 200025, Peoples R China; 9.Natl Facil Prot Sci Shanghai, Zhangjiang Lab, Shanghai 201210, Peoples R China; 10.Chinese Acad Sci, Shanghai Sci Res Ctr, Shanghai 201204, Peoples R China |
推荐引用方式 GB/T 7714 | Lan, Pengfei,Chen, Juan,Shi, Shaohua,et al. Structural insight into precursor tRNA processing by yeast ribonuclease P[J]. SCIENCE,2018,362(6415):657-+. |
APA | Lan, Pengfei.,Chen, Juan.,Shi, Shaohua.,Wu, Jian.,Lei, Ming.,...&Lei, Ming.(2018).Structural insight into precursor tRNA processing by yeast ribonuclease P.SCIENCE,362(6415),657-+. |
MLA | Lan, Pengfei,et al."Structural insight into precursor tRNA processing by yeast ribonuclease P".SCIENCE 362.6415(2018):657-+. |
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