In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates | |
Li, Zhenjian1; Tang, Yannan1,2; Li, Dianfan1 | |
刊名 | CRYSTAL GROWTH & DESIGN |
2018 | |
卷号 | 18期号:4页码:2243-2258 |
关键词 | Serial Femtosecond Crystallography Protein-coupled Receptors Lipid Cubic Phase Amino-acid Transporter X-ray Crystallography Crystal-structure Diacylglycerol Kinase Structural Insights Monoolein/water System Staphylococcus-aureus |
ISSN号 | 1528-7483 |
DOI | 10.1021/acs.cgd.7b01678 |
文献子类 | Article |
英文摘要 | Here we retrospectively report the successful application of the host lipid screening strategy in the in meso crystallization of PlsY, a bacterial glycerol 3-phosphate (G3P) acyltransferase that catalyzes the committed step in phospholipid biosynthesis. Two monoacylglycerols (MAGs) with different chain lengths, namely, the 9.9 MAG (monoolein, nine carbons on either side of the cis-double bond in the acyl chain) and the 7.8 MAG, were the two main host lipids used in this study. The native PlsY crystals growing in 9.9 MAG diffracted to 1.62 angstrom. However, the selenomethioninyl crystals growing in the same host lipid under similar precipitant conditions diffracted poorly (4.3 angstrom). Switching the host lipid to 7.8 MAG dramatically improved crystal quality in both size and diffraction, yielding a 2.0-angstrom data set, with which the structure was solved. Along with low-temperature crystallization, 7.8 MAG was also critical for cocrystallization of PlsY with the unstable lipid substrate, acyl phosphate (acylP). Interestingly, however, 7.8 MAG was unsuitable for PlsY-G3P cocrystallization as multiple crystal forms obtained from an extensive screening did not reveal electron density for this water-soluble substrate. In contrast, 9.9 MAG supported the cocrystallization, yielding a 2.37-angstrom data set for the PlsY-G3P complex. We hypothesize that, under crystallization conditions, G3P binding which occurs at the membrane boundary favors a more rigid mesophase lipid bilayer formed by the longer-chain 9.9 MAG. |
电子版国际标准刊号 | 1528-7505 |
WOS研究方向 | Chemistry, Multidisciplinary ; Crystallography ; Materials Science, Multidisciplinary |
语种 | 英语 |
WOS记录号 | WOS:000429508200036 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3423] |
专题 | 生化所2018年发文 上海生化细胞研究所_上海生科院生化细胞研究所 |
通讯作者 | Li, Dianfan |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Natl Ctr Prot Sci Shanghai,Shanghai Sci Res Ctr, 333 Haike Rd, Shanghai 201210, Peoples R China; 2.Univ Chinese Acad Sci, 333 Haike Rd, Shanghai 201210, Peoples R China |
推荐引用方式 GB/T 7714 | Li, Zhenjian,Tang, Yannan,Li, Dianfan. In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates[J]. CRYSTAL GROWTH & DESIGN,2018,18(4):2243-2258. |
APA | Li, Zhenjian,Tang, Yannan,&Li, Dianfan.(2018).In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates.CRYSTAL GROWTH & DESIGN,18(4),2243-2258. |
MLA | Li, Zhenjian,et al."In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates".CRYSTAL GROWTH & DESIGN 18.4(2018):2243-2258. |
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