alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons | |
Wei, Dan1; Gao, Nannan1; Li, Lei1; Zhu, Jing-Xiang1; Diao, Lei1; Huang, Jiansong1,4; Han, Qing-Jian1; Wang, Shaogang1,4; Xue, Huaqing1; Wang, Qiong1 | |
刊名 | CEREBRAL CORTEX |
2018 | |
卷号 | 28期号:9页码:3332-3346 |
关键词 | Molecular-basis Acetyltransferase Mec-17 Cell Mechanisms Taxol Organization Instability Deficiency Elongation |
ISSN号 | 1047-3211 |
DOI | 10.1093/cercor/bhx225 |
文献子类 | Article |
英文摘要 | Axon growth is tightly controlled to establish functional neural circuits during brain development. Despite the belief that cytoskeletal dynamics is critical for cell morphology, how microtubule acetylation regulates axon development in the mammalian central nervous system remains unclear. Here, we report that loss of a-tubulin acetylation by ablation of MEC-17 in mice predisposes neurons to axon overbranching and overgrowth. Introduction of MEC-17(F183A) lacking alpha-tubulin acetyltransferase activity into MEC-17-deficient neurons failed to rescue axon defects. Moreover, loss of alpha-tubulin acetylation led to increases in microtubule debundling, microtubule invasion into filopodia and growth cones, and microtubule plus-end dynamics along the axon. Taxol application dampened microtubule hyperdynamics and suppressed axon overbranching and overgrowth in MEC-17-deficient neurons. Thus, our study reveals that alpha-tubulin acetylation acts as a brake for axon overbranching and overgrowth by dampening microtubule dynamics, providing insight into the role of microtubule post-translational modifications in regulating neural development. |
电子版国际标准刊号 | 1460-2199 |
WOS研究方向 | Neurosciences |
语种 | 英语 |
WOS记录号 | WOS:000443545600021 |
内容类型 | 期刊论文 |
版本 | 出版稿 |
源URL | [http://202.127.25.143/handle/331003/3377] |
专题 | 生化所2018年发文 |
通讯作者 | Bao, Lan |
作者单位 | 1.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biol Sci,Univ Chinese Acad Sci, State Key Lab Cell Biol,Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Inst Neurosci, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, State Key Lab Neurosci, CAS Ctr Excellence Brain Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
推荐引用方式 GB/T 7714 | Wei, Dan,Gao, Nannan,Li, Lei,et al. alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons[J]. CEREBRAL CORTEX,2018,28(9):3332-3346. |
APA | Wei, Dan.,Gao, Nannan.,Li, Lei.,Zhu, Jing-Xiang.,Diao, Lei.,...&Zhang, Xu.(2018).alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons.CEREBRAL CORTEX,28(9),3332-3346. |
MLA | Wei, Dan,et al."alpha-Tubulin Acetylation Restricts Axon Overbranching by Dampening Microtubule Plus-End Dynamics in Neurons".CEREBRAL CORTEX 28.9(2018):3332-3346. |
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