Direct conversion of shrimp shells to O-acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents

doi:10.1039/c8gc02506a

	Direct conversion of shrimp shells to O-acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents
	Feng, Mi 1,2; Lu, Xingmei 1,2; Zhang, Jie 2; Li, Yi 3; Shi, Chunyan 2; Lu, Lingling 3; Zhang, Suojiang 1,2
刊名	GREEN CHEMISTRY
	2019-01-07
卷号	21 期号:1 页码:87-98
ISSN号	1463-9262
DOI	10.1039/c8gc02506a
英文摘要	To obtain O-acylated chitin from shrimp shells directly, the used solvent should have multiple functions to remove calcium carbonate, protein, and acylated chitin. Herein, we use the acidic natural deep eutectic solvents (NADESs) with the ability to release H+ and various hydrogen bonding sites to achieve the above goal. The involved NADESs with three abilities of decalcification, deproteinization and acylation replaced acids, alkalis, catalysts, and acylation reagents in the conventional method. The experimental results revealed that the components of the NADESs, experiment temperature and time played key roles in the purity and degree of substitution (DS) of O-acylated chitin. Meanwhile, the ratio of shrimp shells to NADESs and a small amount of water had little effect on the preparation of O-acylated chitin. With the optimal NADES (choline chloride/dl-malic acid 1:2, ChCl 1-dl Mal 2) treatment under the optimal conditions, the purity of O-malate chitin reached 98.6% with a DS of 0.46, exhibiting antibacterial and anti-tumor effects. The experimental results showed that the removal of calcium carbonate and protein and acylation of chitin were carried out simultaneously. Mechanistic exploration using spectroscopy and experiments confirmed that H+ release from ChCl 1-dl Mal 2 was the main reason for the removal of calcium carbonate and initiation acylation reaction. The protein was degraded to amino acids because of the acidity of ChCl 1-dl Mal 2 and dissolved in the NADES due to hydrogen bonding formation with ChCl 1-dl Mal 2.
资助项目	National Basic Research Program of China (973 Program)[2015CB251401] ; Natural Scientific Fund of China[21476234] ; Natural Scientific Fund of China[21506231] ; National CAS/SAFEA International Partnership Program for Creative Research Teams[20140491518] ; Key Research Program of Frontier Sciences CAS[QYZDY-SSW-JSC011]
WOS关键词	CHOLINE CHLORIDE ; AQUEOUS-SOLUTION ; LOBSTER SHELLS ; IONIC LIQUIDS ; HIGH-PURITY ; CHITOSAN ; EXTRACTION ; WASTE ; DERIVATIVES ; WATER
WOS研究方向	Chemistry ; Science & Technology - Other Topics
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000454943500008
资助机构	National Basic Research Program of China (973 Program) ; Natural Scientific Fund of China ; National CAS/SAFEA International Partnership Program for Creative Research Teams ; Key Research Program of Frontier Sciences CAS
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/27698]
专题	中国科学院过程工程研究所
通讯作者	Lu, Xingmei; Zhang, Suojiang
作者单位	1.Chinese Acad Sci, Beijing Key Lab Ion Liquids Clean Proc, State Key Lab Multiphase Complex Syst, CAS Key Lab Green Proc & Engn,Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Sch Chem & Engn, Beijing 100049, Peoples R China 3.Capital Med Univ, Beijing Ctr Neural Regenerat & Repair, Key Lab Neurodegenerat Dis,Beijing Inst Brain Dis, Ctr Parkinsons Dis,Dept Neurobiol,Minist Educ, Beijing 100054, Peoples R China
推荐引用方式 GB/T 7714	Feng, Mi,Lu, Xingmei,Zhang, Jie,et al. Direct conversion of shrimp shells to O-acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents[J]. GREEN CHEMISTRY,2019,21(1):87-98.
APA	Feng, Mi.,Lu, Xingmei.,Zhang, Jie.,Li, Yi.,Shi, Chunyan.,...&Zhang, Suojiang.(2019).Direct conversion of shrimp shells to O-acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents.GREEN CHEMISTRY,21(1),87-98.
MLA	Feng, Mi,et al."Direct conversion of shrimp shells to O-acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents".GREEN CHEMISTRY 21.1(2019):87-98.