Enteromorpha prolifera oligomers relieve pancreatic injury in streptozotocin (STZ)-induced diabetic mice

doi:10.1016/j.carbpol.2018.11.019

	Enteromorpha prolifera oligomers relieve pancreatic injury in streptozotocin (STZ)-induced diabetic mice
	Yuan, Xubing 1,2,3; Zheng, Junping 1,2,3; Ren, Lishi 1,2; Jiao, Siming 1,2; Feng, Cui 1,2; Du, Yuguang 1,2; Liu, Hongtao 1,2
刊名	CARBOHYDRATE POLYMERS
	2019-02-15
卷号	206 页码:403-411
关键词	Enteromorpha prolifera oligomers Antioxidation Diabetes mellitus Inflammation Apoptosis
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2018.11.019
英文摘要	The polysaccharides of Enteromorpha prolifera (PEP) displayed various bioactivities such as anti-viral, anti-inflammatory and immune-regulative effects. However, no studies were performed on the biological effect of Enteromorpha prolifera oligomers (EPO). In this study, we prepared EPO and evaluated their anti-diabetic effect By enzymatic degradation, EPO were produced from PEP, and the average molecular weight was identified to be 44.1 kDa by Gel Permeation Chromatography (GPC) analysis. The major monosaccharide units of EPO were measured to be rhamnose, glucuronic acid, glucose, xylose and galactose by capillary electrophoresis assay. Based on the in vitro studies, EPO presented potent reducing power and antioxidant effect such as the scavenging of 1, 1-dipheny1-2-picrylhydrazyl (DPPH), superoxide and NO radicals. The in vivo studies show that EPO relieved the symptoms of polydipsia, polyphagia, emaciation and hyperglycemia in streptozotocin (STZ)-induced diabetic mice to a certain extent Further, by using the quantitative real-time PCR (qPCR) assay and immuno-fluorescence staining, EPO was proved to promote the insulin secretion by reducing pancreatic inflammation and apoptosis in diabetic mice. In summary, our results indicate that the mitigation of EPO on pancreatic damage might be an effective way to ameliorate the diabetes mellitus.
资助项目	National Natural Science Foundation Of China[31570801] ; National Natural Science Foundation Of China[31500747]
WOS关键词	IN-VITRO ANTIOXIDANT ; SULFATED POLYSACCHARIDES ; DEGRADED POLYSACCHARIDES ; PURIFICATION ; INFLAMMATION ; EXTRACTION ; LINZA
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000452982700046
资助机构	National Natural Science Foundation Of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/27613]
专题	中国科学院过程工程研究所
通讯作者	Du, Yuguang; Liu, Hongtao
作者单位	1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Inst Proc Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Xubing,Zheng, Junping,Ren, Lishi,et al. Enteromorpha prolifera oligomers relieve pancreatic injury in streptozotocin (STZ)-induced diabetic mice[J]. CARBOHYDRATE POLYMERS,2019,206:403-411.
APA	Yuan, Xubing.,Zheng, Junping.,Ren, Lishi.,Jiao, Siming.,Feng, Cui.,...&Liu, Hongtao.(2019).Enteromorpha prolifera oligomers relieve pancreatic injury in streptozotocin (STZ)-induced diabetic mice.CARBOHYDRATE POLYMERS,206,403-411.
MLA	Yuan, Xubing,et al."Enteromorpha prolifera oligomers relieve pancreatic injury in streptozotocin (STZ)-induced diabetic mice".CARBOHYDRATE POLYMERS 206(2019):403-411.