Short-chain ketone production by engineered polyketide synthases in Streptomyces albus

	Short-chain ketone production by engineered polyketide synthases in Streptomyces albus
	Baidoo, Edward E. K.; Yuzawa, Satoshi; Mirsiaghi, Mona; Jocic, Renee; Fujii, Tatsuya; Masson, Fabrice; Benites, Veronica T.; Sundstrom, Eric; Tanjore, Deepti; Pray, Todd R.
刊名	NATURE COMMUNICATIONS
	2018
文献子类	期刊论文
英文摘要	Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g . l(-1) of C6 and C7 ethyl ketones and several hundred mg . l(-1) of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/13435]
专题	合成所
推荐引用方式 GB/T 7714	Baidoo, Edward E. K.,Yuzawa, Satoshi,Mirsiaghi, Mona,et al. Short-chain ketone production by engineered polyketide synthases in Streptomyces albus[J]. NATURE COMMUNICATIONS,2018.
APA	Baidoo, Edward E. K..,Yuzawa, Satoshi.,Mirsiaghi, Mona.,Jocic, Renee.,Fujii, Tatsuya.,...&Keasling, Jay D..(2018).Short-chain ketone production by engineered polyketide synthases in Streptomyces albus.NATURE COMMUNICATIONS.
MLA	Baidoo, Edward E. K.,et al."Short-chain ketone production by engineered polyketide synthases in Streptomyces albus".NATURE COMMUNICATIONS (2018).