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	Electrophysiological properties of SF1 neurons in Ventromedial Hypothalamus
	Chen Shanping; Liu Yunhi; Gao Dashuang; Zhang Lu; Zhou Xinyi; Xiao Qian; Hu Nan; Zhang Xinzhou; Tu Jie; Yang Fan
	2018
会议日期	2018
会议地点	San Diego
英文摘要	The Ventromedial Hypothalamus (VMH) is known to regulating feeding, energy homeostasis, emotional state, among other important processes. Previous studies have indicated that most dorsal medial/central VMH (dm/c VMH) express steroidogenic factor 1 (SF1), and established that these neurons are involved in the control of glucose metabolism, bone metabolism and defensive state. It is also known that these functions are mediated by anatomically distinct and/or genetically specified subpopulations of SF1 neurons in dm/c VMH. However, relatively little is known about the electrophysiological heterogeneity of these SF1 neurons that form these distinct subpopulations. Here we describe a clustering-based classification study of dm/c VMH neurons in C57BL 6J mice (3-4 months, male) using whole cell patch-clamp recordings. We found that these neurons markedly vary in their electrophysiological properties, and could be divided into three types: silent (rheobase>20pA), or Type I; spontaneous firing, or Type II; large input resistance and silent (rheobase≤10pA), or Type III. Notably, most Type III neurons exhibited obvious HCN-mediated potential sag, when treated with -100pA current injection. Next, we performed whole-cell recordings to characterize electrophysiological change occurring in dm/c VMH neurons upon chronic stress. The resting potentials of dm/c VMH neurons were, on average, more depolarized in stress mice than those in wild type, and the firing onset time was shorter. Result of clustering analysis suggests that the significant increase in the percentage of spontaneous firing neurons may contribute to these electrophysiological changes. These results demonstrated that clustering is helpful to understand the electrophysiological mechanisms underlie VMH regulating anxiety-like behavior.
语种	英语
内容类型	会议论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/13888]
专题	深圳先进技术研究院_脑所
推荐引用方式 GB/T 7714	Chen Shanping,Liu Yunhi,Gao Dashuang,et al. Electrophysiological properties of SF1 neurons in Ventromedial Hypothalamus[C]. 见:. San Diego. 2018.

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