Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay

	Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay
	Israr, Muhammad; Wiegand, Peter; Kulstein, Galina; Zar, Mian Sahib; Shahid, Ahmad Ali; Shahzad, Muhammad Saqib; Shin, Kyoung-Jin; Lee, Hwan Young; Lee, Sang-Seob
刊名	JOURNAL OF FORENSIC SCIENCES
	2018
文献子类	期刊论文
英文摘要	This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with acertain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14902]
专题	深圳先进技术研究院_其他
推荐引用方式 GB/T 7714	Israr, Muhammad,Wiegand, Peter,Kulstein, Galina,et al. Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay[J]. JOURNAL OF FORENSIC SCIENCES,2018.
APA	Israr, Muhammad.,Wiegand, Peter.,Kulstein, Galina.,Zar, Mian Sahib.,Shahid, Ahmad Ali.,...&Lee, Sang-Seob.(2018).Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay.JOURNAL OF FORENSIC SCIENCES.
MLA	Israr, Muhammad,et al."Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay".JOURNAL OF FORENSIC SCIENCES (2018).