Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles

	Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles
	Mingbin Zheng; Xiujun Fan; Lintao Cai; Baozheng Zhang
刊名	Jove-Journal of Visualized Experiments
	2018
文献子类	期刊论文
英文摘要	An effective cancer therapeutic method reduces and eliminates tumors with minimal systemic toxicity. Actively targeting nanoparticles offer a promising approach to cancer therapy. The glycosaminoglycan placental chondroitin sulfate A (plCSA) is expressed on a wide range of cancer cells and placental trophoblasts, and malarial protein VAR2CSA can specifically bind to plCSA. A reported placental chondroitin sulfate A binding peptide (plCSA-BP), derived from malarial protein VAR2CSA, can also specifically bind to plCSA on cancer cells and placental trophoblasts. Hence, plCSA-BP-conjugated nanoparticles could be used as a tool for targeted drug delivery to human cancers and placental trophoblasts. In this protocol, we describe a method to synthesize plCSA-BP-conjugated lipid-polymer nanoparticles loaded with doxorubicin (plCSA-DNPs); the method consists of a single sonication step and bioconjugate techniques. In addition, several methods for characterizing plCSA-DNPs, including determining their physicochemical properties and cellular uptake by placental choriocarcinoma (JEG3) cells, are described.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14694]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Mingbin Zheng,Xiujun Fan,Lintao Cai,et al. Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles[J]. Jove-Journal of Visualized Experiments,2018.
APA	Mingbin Zheng,Xiujun Fan,Lintao Cai,&Baozheng Zhang.(2018).Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles.Jove-Journal of Visualized Experiments.
MLA	Mingbin Zheng,et al."Synthesis and characterization of Plancental Chondroitin Sulfate A(plCSA)-Targeting Lipid-Polymer Nanoparticles".Jove-Journal of Visualized Experiments (2018).