siRNA-mediated &ITc-Rel&IT knockdown ameliorates collagen-induced arthritis in mice.

	siRNA-mediated &ITc-Rel&IT knockdown ameliorates collagen-induced arthritis in mice.
	Fan, Tingting; Zhong, Fuhua; Liu, Ruiling; Chen, Youhai H.; Wang, Ting; Ruan, Qingguo
刊名	INTERNATIONAL IMMUNOPHARMACOLOGY
	2018
文献子类	期刊论文
英文摘要	Previous studies have shown that inflammatory mediators involved in the development of rheumatoid arthritis (RA) are regulated by the Rel/nuclear factor-kappa B (Rel/NF-kappa B) transcription factor family. c-Rel, a member of the Rel/NF-kappa B family that is preferentially expressed by immune cells, is a risk factor for several inflammatory diseases including RA. In the current study, we investigated whether targeting c-Rel can be used to treat col-lagen-induced arthritis, an animal model for RA. c-Rel specific siRNA (siRel) delivered by nanoparticles was used to knockdown the expression of c-Rel. Our results showed that siRel treatment significantly ameliorated col-lagen-induced arthritis. Further study revealed that c-Rel expression in the dendritic cells and macrophages from mice treated with siRel was significantly down-regulated. Consistent with the phenotypical result, the expression of inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IL-12 and IL-23 by peritoneal macrophages and splenocytes were significantly decreased. In addition, attenuated systemic and collagen-specific Th1 and Th17 immune responses were observed. Furthermore, we found that the expression of inflammatory cytokines was significantly down-regulated and the infiltration of CD3(+) T cells and F4/80(+) macrophages was markedly reduced in hind paws of mice treated with siRel. Collectively, our study provides strong evidence that siRNA-mediated c-Rel knockdown can suppress the development of collagen-induced arthritis in mice. Therefore, blocking c-Rel may represent an attracting strategy for the treatment of human rheumatoid arthritis.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14630]
专题	深圳先进技术研究院_医药所
推荐引用方式 GB/T 7714	Fan, Tingting,Zhong, Fuhua,Liu, Ruiling,et al. siRNA-mediated &ITc-Rel&IT knockdown ameliorates collagen-induced arthritis in mice.[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2018.
APA	Fan, Tingting,Zhong, Fuhua,Liu, Ruiling,Chen, Youhai H.,Wang, Ting,&Ruan, Qingguo.(2018).siRNA-mediated &ITc-Rel&IT knockdown ameliorates collagen-induced arthritis in mice..INTERNATIONAL IMMUNOPHARMACOLOGY.
MLA	Fan, Tingting,et al."siRNA-mediated &ITc-Rel&IT knockdown ameliorates collagen-induced arthritis in mice.".INTERNATIONAL IMMUNOPHARMACOLOGY (2018).