Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL

	Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL
	Zheng, Hairong; Li, Yuezhou; Duan, Shumin; Ye, Jia; Tang, Siyang; Meng, Long; Li, Xia; Wen, Xiaoxu; Chen, Sihan; Niu, Lili
刊名	NANO LETTERS
	2018
文献子类	期刊论文
英文摘要	Externally controlling the excitation of a neuronal subset through ion channels activation can modulate the firing pattern of an entire neural circuit in vivo. As nanovalves in the cell membrane, ion channels can be opened by light (optogenetics) or ultrasonic (sonogenetics) means. A thoroughly analyzed force sensor is the Escherichia coli mechanosensitive channel of large conductance (MscL). Here we expressed MscL in rat hippocampal neurons in a primary culture and showed that it could be activated by low-pressure ultrasound pulses. The gain-of-function mutation, I92L, sensitized MscL's sonic response, triggering action potentials at a peak negative pressure as low as 0.25 MPa. Further, the I92L MscL reliably elicited individual spikes by timed brief pulses, making excitation programmable. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/14340]
专题	深圳先进技术研究院_医工所
推荐引用方式 GB/T 7714	Zheng, Hairong,Li, Yuezhou,Duan, Shumin,et al. Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL[J]. NANO LETTERS,2018.
APA	Zheng, Hairong.,Li, Yuezhou.,Duan, Shumin.,Ye, Jia.,Tang, Siyang.,...&Shu, Qiang.(2018).Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.NANO LETTERS.
MLA	Zheng, Hairong,et al."Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL".NANO LETTERS (2018).