An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 土壤环境科学实验室

	An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China
	Li, Hu; Zhu, Yong-Guan; Zhu, Gui-Bing; Yu, Shen; Chen, Yong-Shan; Li, Hong; Marshall, Christopher W.; Weng, Bo-Sen; Yang, Xiao-Ru
刊名	JOURNAL OF SOILS AND SEDIMENTS
	2017-10-01
卷号	17 期号:10 页码:2537-2546
关键词	Activity Anammox Estuarine Sediments n Loss Spatial Variation
英文摘要	Purpose Despite its importance, anammox (anaerobic ammonium oxidation) in estuarine sediment systems remains poorly understood, particularly at the continental scale. This study aimed to understand the abundance, diversity, and activity of anammox bacteria and to determine the main factors influencing the anammox process in estuarine sediments in China. Materials and methods Estuarine sediments were collected from 18 estuaries spanning over 4000 km. Experiments using an (15)Ntracer, quantitative PCR, and clone library construction were used to determine the activity, abundance, and diversity of anammox bacteria. The impact of environmental factors on anammox processes was also determined. Results and discussion The abundance of the anammox-specific hydrazine synthase (hzsB) gene ranged from 1.8 x 105 +/- 3.4 x 104 to 3.6 x 108 +/- 7.5 x 107 copies g(-1) dw. Candidatus Scalindua, Brocadia, Kuenenia, Jettenia, and two novel unidentified clusters were detected, with Scalindua dominating the anammox population. Additionally, the abundances of Scalindua, Kuenenia, and Brocadia were found to be significantly correlated with latitude. The anammox rates ranged from 0.29 +/- 0.15 to 13.68 +/- 3.98 nmol N g(-1) dw h(-1) and contributed to 2.39-82.61% of total N-2 production. Pearson correlation analysis revealed that the anammox rate was positively correlated with total nitrogen, total carbon, and temperature, and was negatively correlated with dissolved oxygen (DO). The key factors influencing the hzsB gene abundance were ammonium concentration, salinity, and DO. Ammonium concentration, pH, temperature, and latitude were main variables shaping the anammox-associated bacterial community. Conclusions Our results suggested that anammox bacteria are ubiquitous in coastal estuaries in China and underline the importance of anammox resulting in N loss at a continental scale.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39577]
专题	生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Li, Hu,Zhu, Yong-Guan,Zhu, Gui-Bing,et al. An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China[J]. JOURNAL OF SOILS AND SEDIMENTS,2017,17(10):2537-2546.
APA	Li, Hu.,Zhu, Yong-Guan.,Zhu, Gui-Bing.,Yu, Shen.,Chen, Yong-Shan.,...&Yang, Xiao-Ru.(2017).An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China.JOURNAL OF SOILS AND SEDIMENTS,17(10),2537-2546.
MLA	Li, Hu,et al."An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China".JOURNAL OF SOILS AND SEDIMENTS 17.10(2017):2537-2546.