Cleaning procedure for protein G affinity columns

	Cleaning procedure for protein G affinity columns
	Huang, JX; Yan, Z
刊名	JOURNAL OF IMMUNOLOGICAL METHODS
	2000
卷号	237 期号:2015-01-02 页码:203-205
关键词	Protein g Affinity Perfusion
DOI	10.1016/S0022-1759(00)00133-2
英文摘要	An efficient method for cleaning protein G affinity columns is described in which impurities are removed effectively with a combination of 4 mol/1 urea and 0.1 mol/1 sodium hydroxide. These cleaning conditions have only a minor deleterious effect on the binding capacity of the column and no effect on its selectivity for IgG. This cleaning procedure should prolong the life of costly affinity columns used in the purification of immunoglobulin G (IgG). (C) 2000 Published by Elsevier Science B.V. All rights reserved.
WOS记录号	WOS:000086212100018
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/24177]
专题	生态环境研究中心_水污染控制实验室
推荐引用方式 GB/T 7714	Huang, JX,Yan, Z. Cleaning procedure for protein G affinity columns[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2000,237(2015-01-02):203-205.
APA	Huang, JX,&Yan, Z.(2000).Cleaning procedure for protein G affinity columns.JOURNAL OF IMMUNOLOGICAL METHODS,237(2015-01-02),203-205.
MLA	Huang, JX,et al."Cleaning procedure for protein G affinity columns".JOURNAL OF IMMUNOLOGICAL METHODS 237.2015-01-02(2000):203-205.