LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules

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	LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules
	Hakoyama, Tsuneo; Duan, Guilan; Kamiya, Takehiro; Miwa, Hiroki; Lombardo, Fabien; Sato, Shusei; Tabata, Satoshi; Chen, Zheng; Watanabe, Toshihiro; Shinano, Takuro
刊名	PLANT JOURNAL
	2017-06-01
卷号	90 期号:6 页码:1108-1119
关键词	Molybdenum Molybdate Transporter Lotus Japonicus Nodule Nitrogen Fixation
英文摘要	Molybdenum (Mo) is an essential nutrient for plants, and is required for nitrogenase activity of legumes. However, the pathways of Mo uptake from soils and then delivery to the nodules have not been characterized in legumes. In this study, we characterized a high-affinity Mo transporter (LjMOT1) from Lotus japonicus. Mo concentrations in an ethyl methanesulfonate-mutagenized line (ljmot1) decreased by 70-95% compared with wild-type (WT). By comparing the DNA sequences of four AtMOT1 homologs between mutant and WT lines, one point mutation was found in LjMOT1, which altered Trp(292) to a stop codon; no mutation was found in the other homologous genes. The phenotype of Mo concentrations in F-2 progeny from ljmot1 and WT crosses were associated with genotypes of LjMOT1. Introduction of endogenous LjMOT1 to ljmot1 restored Mo accumulation to approximately 60-70% of the WT. Yeast expressing LjMOT1 exhibited high Mo uptake activity, and the K-m was 182 nM. LjMOT1 was expressed mainly in roots, and its expression was not affected by Mo supply or rhizobium inoculation. Although Mo accumulation in the nodules of ljmot1 was significantly lower than that of WT, it was still high enough for normal nodulation and nitrogenase activity, even for cotyledons-removed ljmot1 plants grown under low Mo conditions, in this case the plant growth was significantly inhibited by Mo deficiency. Our results suggest that LjMOT1 is an essential Mo transporter in L. japonicus for Mo uptake from the soil and growth, but is not for Mo delivery to the nodules.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39049]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Hakoyama, Tsuneo,Duan, Guilan,Kamiya, Takehiro,et al. LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules[J]. PLANT JOURNAL,2017,90(6):1108-1119.
APA	Hakoyama, Tsuneo.,Duan, Guilan.,Kamiya, Takehiro.,Miwa, Hiroki.,Lombardo, Fabien.,...&Fujiwara, Toru.(2017).LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules.PLANT JOURNAL,90(6),1108-1119.
MLA	Hakoyama, Tsuneo,et al."LjMOT1, a high-affinity molybdate transporter from Lotus japonicus, is essential for molybdate uptake, but not for the delivery to nodules".PLANT JOURNAL 90.6(2017):1108-1119.