Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence

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	Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence
	Wang, Zijian; Yang, Lihua; Zha, Jinmiao
刊名	CHEMOSPHERE
	2017
卷号	166 期号:0 页码:174-183
关键词	Pentachlorophenol Molecular Docking Chinese Rare Minnow (Gobiocypris Rarus) Reproductive System Interrenal System
英文摘要	The present study investigated the effects on reproductive and interrenal system by pentachlorophenol (PCP) using in silico and in vivo assays. Molecular docking results indicated interacting potency of PCP with steroid receptors (ER alpha, ER beta, AR, GR) but not Cytochrome P450 enzymes (CYPs). In the in vivo assay, sexually matured rare minnow (Gobiocypris rams) was exposed to environmental relevant concentrations of PCP (0, 0.5, 5, 50 mu g L-1). In male fish, 14-d exposure caused up-regulation of mRNA levels of hepatic er alpha, er beta, ar, gr, vtg and gonadal er alpha, vtg, ar, dmrt1, providing evidence for agonistic activities for steroid receptors by PCP. The up-regulated mRNA of gnrh, crf, pomc in the brain also indicated feed forward responses of the hypothalamic-pituitary-gonadal/interrenal (HPG/I) axis. However, at 28th d the feed-forward response of the HPG axis seemed eased back and the HPI axis showed negative feedback responses. Corresponding changes including increases of plasma steroid hormones, inhibition of spermatogenesis, and decreased RSI were observed in male fish upon 28-d exposure to PCP. In the females, a transition from feed-forward responses to negative feedbacks of the HPG/I axis was also indicated by the transcriptional profiles at 14th and 28th day. Corresponding changes including increased E2, T and decreased C levels, degenerated ovaries, and decreased GSI and RSI were also observed. Overall, we concluded that PCP could interfere with steroid receptors, evoke responses of HPG/I axis, and finally result in adverse effects on reproductive and interrenal system in rare minnow at environmental relevant concentrations. (C) 2016 Elsevier Ltd. All rights reserved.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39540]
专题	生态环境研究中心_环境水质学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Zijian,Yang, Lihua,Zha, Jinmiao. Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence[J]. CHEMOSPHERE,2017,166(0):174-183.
APA	Wang, Zijian,Yang, Lihua,&Zha, Jinmiao.(2017).Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence.CHEMOSPHERE,166(0),174-183.
MLA	Wang, Zijian,et al."Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence".CHEMOSPHERE 166.0(2017):174-183.