S-Adenosyl-L-methionine production by Saccharomyces cerevisiae SAM 0801 using DL-methionine mixture: From laboratory to pilot scale

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	S-Adenosyl-L-methionine production by Saccharomyces cerevisiae SAM 0801 using DL-methionine mixture: From laboratory to pilot scale
	Ren, Wenqiang; Cai, Di; Hu, Song; Xia, Shasha; Wang, Zheng; Tan, Tianwei; Zhang, Qinghua
刊名	PROCESS BIOCHEMISTRY
	2017-03-01
卷号	62 期号:0 页码:48-52
关键词	S-adenosyl-l-methionine Fermentation Saccharomyces Cerevisiae Dl-methionine
英文摘要	This study sought to develop a cost-effective biological catalysis process for S-adenosyl-L-methionine (SAM) production. During the process, a mixture of (D)-methionine ((D)-Met) and (L)-methionine (L-Met), namely (DL)-Met, was used as substrate to replace the conventional but expensive pure (L)-Met. The concentration of DL-Met in substrate was optimized. When 80 g/L of (DL)-Met was added in a 5 L-scale bioreactor, 13.74 g/L of SAM was produced with an (L)-Met conversion rate of 32.15%. The fermentation process was then scaled up to meet the requirements of realistic industrial SAM production. In a 300 L-scale fermentation process, 10.45 g/L of SAM was achieved, with an (L)-Met conversion rate of 32.61%. Moreover, the proportion of (D)-Met remaining in broth increased from 50% at the beginning to 76.89% at the end of fermentation. The fermentation process is generally appropriate for commercial SAM production.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39407]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Ren, Wenqiang,Cai, Di,Hu, Song,et al. S-Adenosyl-L-methionine production by Saccharomyces cerevisiae SAM 0801 using DL-methionine mixture: From laboratory to pilot scale[J]. PROCESS BIOCHEMISTRY,2017,62(0):48-52.
APA	Ren, Wenqiang.,Cai, Di.,Hu, Song.,Xia, Shasha.,Wang, Zheng.,...&Zhang, Qinghua.(2017).S-Adenosyl-L-methionine production by Saccharomyces cerevisiae SAM 0801 using DL-methionine mixture: From laboratory to pilot scale.PROCESS BIOCHEMISTRY,62(0),48-52.
MLA	Ren, Wenqiang,et al."S-Adenosyl-L-methionine production by Saccharomyces cerevisiae SAM 0801 using DL-methionine mixture: From laboratory to pilot scale".PROCESS BIOCHEMISTRY 62.0(2017):48-52.