Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS
	Wang, Hailin; Zhao, Shuli; Xu, Tian; Yin, Junfa; Liu, Baodong; Wu, Danni; Xu, Qun; Chen, Xi
刊名	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
	2017-05-18
卷号	1064 期号:0 页码:1-6
关键词	Ultra-performance Liquid Chromatography Tandem - Mass Spectrometry (Uhplc-ms/ms) 8-hydroxydeoxyguanosine (8-ohdg) Peripheral Blood Leukocyte Oxidative Dna Damage
英文摘要	8-Hydroxydeoxyguanosine (8-OHdG) is a widely-used biomarker of oxidative DNA damages. 8-OHdG in peripheral blood leukocyte is associated with mutation and cancer risk. The level of 8-OHdG in peripheral blood leukocytes can indicate a long-term response to oxidative stress rather than that in urine. Accurate identification and quantification of leukocyte 8-OHdG are essential for understanding its mechanism of formation, repair, and biological consequences. In this study, a fast and accurate ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated to detect 8-OHdG in human peripheral leukocyte. DNA in blood samples were extracted and digested, then subjected onto UHPLC-MS/MS using an isocratic elution on a Zorbax Eclipse Plus C18 column (2.1 x 100 mm, 1.8 mu m). Multiple reaction monitoring (MRM) mode was adopted by using [N-15(5)]-8-OHdG as an internal standard. The assay was linear over the concentration range of 1.0-100 nM with R-2 = 0.999. The accuracy for spiked samples was 90.9%-94.8%, and the intra-day precision was within 3.7%. The limit of detection (LOD) is 0.30 nM and limit of quantification (LOQ) is 1.0 nM with 5 mu l of sample injection. By the analysis of human leukocyte 8-OHdG (n = 121) using the developed UHPLC-MS/MS method, it demonstrates that the level of leukocyte 8-OHdG of the cancer patients group (n = 46) is significantly higher than that of the health control (n = 75).
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/39285]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Wang, Hailin,Zhao, Shuli,Xu, Tian,et al. Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1064(0):1-6.
APA	Wang, Hailin.,Zhao, Shuli.,Xu, Tian.,Yin, Junfa.,Liu, Baodong.,...&Chen, Xi.(2017).Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1064(0),1-6.
MLA	Wang, Hailin,et al."Detection of 8-hydroxydeoxyguanosine (8-OHdG) as a biomarker of oxidative damage in peripheral leukocyte DNA by UHPLC-MS/MS".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1064.0(2017):1-6.