Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics

doi:10.3389/fmicb.2017.01406

	Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics
	Wang, SC (Wang, Shaochen)1; Gao, X (Gao, Xia)1; Gao, YJ (Gao, Yuejiao)1; Li, YQ (Li, Yanqing)1; Cao, MM (Cao, Mingming)1; Xi, ZH (Xi, Zhenhua)2; Zhao, LX (Zhao, Lixing)3; Feng, ZY (Feng, Zhiyang)1; Feng, ZY
刊名	FRONTIERS IN MICROBIOLOGY
	2017
卷号	8 期号:0 页码:1406
关键词	Antibiotic-resistance Drug-resistance Resistome Determinant Bacteria Reveals Poultry Update Efflux Food
DOI	10.3389/fmicb.2017.01406
文献子类	Article
英文摘要	Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic resistances that may transfer among clinical and commensal microorganisms. To investigate tetracycline resistance genes from soil bacteria in different habitats, we performed functional analysis of three metagenomic libraries derived from soil samples collected from Yunnan, Sichuan, and Tibet, respectively, in China. We found efflux transporter genes form all the libraries, including 21 major facilitator superfamily efflux pump genes and one multidrug and toxic compound extrusion (MATE) transporter gene. Interestingly, we also identified two tetracycline destructase genes, belonging to a newly described family of tetracycline-inactivating enzymes that scarcely observed in clinical pathogens, from the Tibet library. The inactivation activity of the putative enzyme was confirmed in vitro by biochemical analysis. Our results indicated that efflux pumps distributed predominantly across habitats. Meanwhile, the mechanism of enzymatic inactivation for tetracycline resistance should not be neglected and merits further investigation.
学科主题	普通生物学
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000406337800001
内容类型	期刊论文
源URL	[http://ir.itpcas.ac.cn/handle/131C11/8024]
专题	青藏高原研究所_图书馆
通讯作者	Feng, ZY
作者单位	1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China. 2.Chinese Acad Sci, Inst Tibetan Plateau Res, Beijing, Peoples R China. 3.Yunnan Univ, Yunnan Inst Microbiol, Kunming, Yunnan, Peoples R China.
推荐引用方式 GB/T 7714	Wang, SC ,Gao, X ,Gao, YJ ,et al. Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics[J]. FRONTIERS IN MICROBIOLOGY,2017,8(0):1406.
APA	Wang, SC .,Gao, X .,Gao, YJ .,Li, YQ .,Cao, MM .,...&Feng, ZY.(2017).Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics.FRONTIERS IN MICROBIOLOGY,8(0),1406.
MLA	Wang, SC ,et al."Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics".FRONTIERS IN MICROBIOLOGY 8.0(2017):1406.