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	Chitin oligosaccharides production fromenzymatic hydrolyzing crab shells
	Wu YY(吴媛媛) ; Lv Y(吕杨) ; Bai XF(白雪芳) ; Du YG(杜昱光)
	2008-10-12
会议名称	the 13th international biotechnology symposium
会议日期	2008-10-12
会议地点	中国
页码	s586/2
通讯作者	杜昱光
中文摘要	in order to find an environment friendly way of producing chitin oligosaccharides from pretreated crab shells, we hydrolyze emulsified pretreated crab shells with chitinase whose gene is from aspergillus fumigatus yj-407 and cloned and expressed in e. coli (xiaet al., 2001; anonymous, 2008). after optimizing chitinase enzymatichydrolysis conditions using orthogonal experimental design, we conduct the hydrolysis experiment under the conditions which is adding 5 g chitinase to every 0.022 g emulsified crab shells and keeping this 2ml solution in 50 ◦c and ph 5.0 environment. after 75 min reaction, we analyze the hydrolyzed products by hpaecpad (anonymous, 2008) and tlc (anonymous, 2008). the results showed that crab shells without fierce acid and alkali pretreatment can be readily hydrolyzed by this chitinase. there are mainly three kinds of chitin oligosaccharides in the hydrolyzed solution. they are n-acetyl-glucosamine, chitobiose and chitotriose, and chitobiose is the most abundant product. the production efficiencies are separately 7.21%, 1.15%, and 0.29% for chitobiose, n-acetyl-glucosamine and chitotriose. the result can be used as a base for the more valuable development about the trash crab shells.
会议主办者	中国科学院 中国工程院 中华人民共和国教育部 中华人民共和国科学技术部 中华人民共和国国家发展改革委员会
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/113242]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Wu YY,Lv Y,Bai XF,et al. Chitin oligosaccharides production fromenzymatic hydrolyzing crab shells[C]. 见:the 13th international biotechnology symposium. 中国. 2008-10-12.

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